Primary GBM cells support productive HCMV replication in vitro.

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Primary GBM cells support productive HCMV replication in vitro. Primary GBM cells support productive HCMV replication in vitro. (A) RNA isolated from GBM 112 and GBM 114 cells infected at the indicated MOI with HCMV TB40/E-mCherry for 72 h was analyzed by RT-PCR for the indicated genes. Mock-infected cells (M) were used as the control. The image is representative of three biological replicates. (B) Supernatants collected at the indicated time (in hours postinfection [hpi]) from GBM 112 and GBM 114 cells infected with HCMV TB40/E-mCherry at the indicated MOI were subjected to a standard plaque assay. Duplicate biological assays were performed. (C) Progeny virus from cell-free or cell-associated samples of GBM 112 or GBM 114 cells infected at a MOI of 1.0 for 144 h were quantitated by a plaque assay. Thee biological replicate experiments were performed and analyzed by Student’s t test for statistical significance. **, P < 0.01; ***, P < 0.001. Che Liu et al. mSphere 2017; doi:10.1128/mSphere.00137-17