Control of bean thrips in navel oranges using ethyl formate fumigation for export to Australia Beth Mitcham , Veronique Bikoba Bill Biasi, and Francine Pupin Department of Plant Sciences University of California, Davis Joseph Morse, UC Riverside In cooperation with: Kathie Blyskal and Alfred Hanson, Sunkist Growers The Linde Group
Ethyl Formate Natural plant volatile Registered for use in Australia and New Zealand by Linde Corporation as VapormateTM in formulation with CO2 Previously registered in US for control of insect pests in raisins, but registration expired in the 1980’s Registration is being pursued by Linde Corporation
VapormateTM Fumigation Laboratory System for VapormateTM Fumigation
Laboratory Fumigation System
Mortality of Bean Thrips after 1 hour VapormateTM Fumigation of Navel Oranges Lab Tests Treatment Rep Conc. EF (%) Untreated Control Vapormate 1 1.5% n =66 100% n = 177 2 0% n = 50 n = 115 3 14.5% n = 62 n = 320
Lab Test Methods 1 hour fumigation with 1% Vapormate at 50°F Evaluations: Initial after 3 wks storage @ 41°F after 5 days @ 55°F after 2 days @ 68°F
Laboratory Test
Laboratory Test 1=none; 2=very slight; 3= slight damage; 4= moderate damage
No damage (1) Very slight damage (2) Slight damage (3) Moderate damage (4) Severe damage (5) Very severe damage (6)
Orange Cove/Sanger Navels-Lab Quality Evaluated after 3 wks @ 41F plus 5 days @ 55F & 2 days @ 68F Untreated Control Fumigate Before Storage Fumigate After Storage
Quality Evaluated after 3 wks @ 41F Gillete Navels-Lab Quality Evaluated after 3 wks @ 41F plus 5 days @ 55F & 2 days @ 68F Untreated Control Fumigate Before Storage Fumigate After Storage
Harding & Legget Navels-Lab Quality Evaluated after 3 wks @ 41F plus 5 days @ 55F & 2 days @ 68F Untreated Control Fumigate Before Storage Fumigate After Storage
Small Degreening Rooms at Sunkist Growers
Semi-Commercial Test Method 1% Vapormate for 1 hour at 50°F Evaluations: Initial after 3 wks storage @ 41°F after 5 days @ 55°F after 2 days @ 68°F
Mortality of Bean Thrips after 1 hour Fumigation with VapormateTM Sunkist Test Conc. EF (%) Untreated Control Vapormate 1 13.7% n = 145 100% n = 417
Sunkist Test
Sunkist Test 1=none; 2=very slight; 3= slight damage; 4= moderate damage
Villa Park – Semi-Commercial Test Quality Evaluated after 3 wks @ 41F plus 5 days @ 55F & 2 days @ 68F Untreated Control Fumigate Before Storage Fumigate After Storage
San Antonio – Semi-Commercial Test Quality Evaluated after 3 wks @ 41F plus 5 days @ 55F & 2 days @ 68F Untreated Control Fumigate Before Storage Fumigate After Storage
Golden Valley – Semi-Commercial Test Quality Evaluated after 3 wks @ 41F plus 5 days @ 55F & 2 days @ 68F Untreated Control Fumigate Before Storage Fumigate After Storage
Mandarin Lab Test Methods to Determine Fruit Tolerance 1 hour fumigation with 1.5% Vapormate at 50°F 8 replications of control and 16 replications for Vapormate trt. 3 weeks shelf life at 68°F with weekly visual evaluation
Tolerance to Vapormate (1.5%) W. Murcott Mandarin Tolerance to Vapormate (1.5%)
Year 3 Commercial trials Treat fruit in packed boxes with static fumigation in marine container Confirm bean thrips mortality with large number of insects Confirm fruit tolerance with many grower sources Develop mortality curves for adult bean thrips Prepare report for APHIS
Vapormate Delivery System
Potential for Vapormate Needs to be registered in U.S. or Needs to be approved for bean thrips control in Australia and New Zealand Already registered there Develop efficacy data for review True of other alternative fumigants as well. GRAS compound Effective against Light Brown Apple Moth
Mortality of the California Red Scale (Aonidiella aurantii) 3 weeks after a 3-hour Fumigation with Ethyl Formate EF concentration (%) Instar 0.75% 1.5% 3.0% First 99.8 100 Second 98.8 Third 99.9 Mature third 99.5 Y. Aharoni, Y. Nitzan, and A. Copel. (1987) Tropical Science (27) 155-157.
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