Biochemistry Lecture 1.

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Presentation transcript:

Biochemistry Lecture 1

Bloom’s Taxonomy Richard C. Overbaugh, Lynn Schultz Old Dominion University

Student Objectives for this course Calculate bioenergetic parameters and evaluate carbon molecules reactions Reproduce and explain key metabolic processes: glycolysis, TCA cycle, electron transport and oxidative phosphorylation, fatty acid oxidation Analyze protein structure and function and evaluate different methods used to assess and test structure and function Trace key metabolites through key pathways, design experiments to test carbon flux Compare and contrast modes of metabolism regulation and judge the effects of different modes

Biochemistry is the chemistry of Living Systems The Chemistry of Carbon and Water Themes for this course: The transformation of energy Levels of complexity

Cells

Cells

Levels of Complexity Lipids

The Inner Life of the Cell http://multimedia.mcb.harvard.edu/anim_innerlife.html

Why Carbon What can we learn from this? Bond Strength (kJ/mol) C – C 347 – 356 C = C 611 837 C – O 336 C – H 356 – 460 Si – Si 230 Si – O 368 O – O 146 O = O 498 N – N 163 N = N 418 946 What can we learn from this? C – C bond is stronger than C – O Stable in oxygen rich environment! Two C – C bonds are stronger than one C = C Chains are stable! C – H bond is strong Hydrocarbons stable at room temperature!

Important Functional Groups Alcohol Thiol Amine Ether Thioether Peroxide Disulfide Aldehyde Ketone Carboxylic acid Ester Anhydride Amide Thioester Phosphate Phosphoester Phosphoanhydride

Carbon and Functional Groups

Other biomolecules PEP NADP+ Phosphatidylcholine

Bioenergetics Cell Reactants Products Steady State = constant flux Structural differences between reactants and products Concentration differences between reactants and products

Water

Hydrogen Bonds

Water

Colligative Properties

pH pH = -log[H+]

Acids

Buffers

Hendeson Hasselbalch Equation  HA H+ + A- 

Summary Biochemistry is the chemistry of living things Which is the chemistry of carbon and water Carbons unique bonding properties Water: hydrogen bonds and ionization Buffers and pH