Microbiology 156 http://chsweb.lr.k12.nj.us/psidelsky Mrs. Sidelsky psidelsky@lrhsd.org psidelsky@bcc.edu.

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Presentation transcript:

Microbiology 156 http://chsweb.lr.k12.nj.us/psidelsky Mrs. Sidelsky psidelsky@lrhsd.org psidelsky@bcc.edu

Class One Please sign safety forms Make sure you understand the safety rules Be able to identify safety equipment in the room

Dress for Laboratory

Laboratory Laboratory coat is required Hair should be tied back No open shoes( sandals) No Gum, food, beverages Keep personal belongings on shelves at the entrance to the laboratory Prepare for each class by reading laboratory experiment(s) for class

Journal For the second class you will need a Journal The Journal can be a marble notebook or spiral bound notebook. It will consist of laboratory experiments for each class. Directions for your laboratory manual will be provided during the second class

Compound Light Microscope

Introducing your microscope Always clean lenses before starting Always with both hands supporting base Store with arm facing the door. Cover Cleaning lenses before and after using Focus with scanning and low before proceeding to high and oil Learn the most effective ways to regulate and manage light

Objective Lenses Objective Lens Mag Total Mag Color of Lens Use Scaniing 5 50 Red Observe the entire slide. Focus with coarse adjustment Low 10 100 Yellow Tweak focus with coarse. Make sure the image is clear and centered High 40 400 Blue Focus only with fine adjustment Oil 1000 White Apply oil and then turn high power objective lens and focus with fine adj

Microscope terms Microscope terms Magnification Total magnification Resolution Depth of field Size of field Working distance Parfocal Parcentric

Microscope and Slides Three Threads Place slide on mechanical stage. Make sure that stage clips are holding slide securely. Use the scanning lens( red) to find the point at which the threads cross. Focus the microscope . Using the coarse adjustment Observe and draw Change to low power. Turn the revolving nose piece so that the yellow lens is in place. Observe and draw

Observations What do you notice about the size of field????? What do you notice about your ability to focus. Focus up and down very carefully and slowly with the fine adjustment What occurs? Why

High Power If your focus is sharp and clear, turn the revolving nosepiece to blue Use the fine adjustment, focus carefully – you are very close to the slide. Observe and draw

Bacterial Slide Take a slide of a bacillus from the supply area Place it on the Stage Repeat the procedure, for focusing Adjust the light and draw Observe and draw.

Bacterial shapes

Bacterial Shapes and Arrangement Streptococci

Rods- Bacilli – Stained with Gram Stain