Agilent 6410 Triple Quad LC/MS Software Mass Hunter QQQ Quantitative Analysis Software Sensitivity, Reliability, Value Whether you need to quantitate drug metabolites, measure herbicide levels in foods, or determine contaminant levels in ground water, the triple quadrupole mass spectrometer has proven itself to be without equal for quantitating trace organic compounds in dirty matrices. The Agilent 6410 Triple Quadrupole LC/MS establishes a new standard for value in a triple quadrupole mass spectrometer; delivering outstanding sensitivity, great ease of use, and legendary Agilent reliability: all at a very attractive price. Features Femtogram-level sensitivity - All ion-path components are designed to maximize ion generation and transmission across a broad mass range-for low limits of detection regardless of application. Fast, high-throughput MS/MS - The high-frequency hexapole collision cell uses linear acceleration to provide very fast MS/MS experiments without cross-experiment memory effects. Sensitivity remains excellent even with 5-ms dwell times. Rugged, reliable performance - Agilent's patented orthogonal spray ion source and high-capacity counter-current drying gas maximize performance across the widest range of flow rates and mobile phases, keep the ion optics cleaner, and reduce background. Ion source choices maximize versatility - The best selection of ion sources in the LC/MS industry-including the revolutionary multimode sources with true simultaneous ESI and APCI-allow the 6410 to address a wide range of applications New software increases throughput - All-new software maximizes convenience and throughput with features like automated tuning, parameterless integration, dynamically linked data, Curve-Fit Assistant, batch review, color-coded outlier flagging, and customizable reporting. Agilent Technologies MassHunter LC-MS/MS Quantitative Analysis Software

Agilent Triple (QqQ) 6410 LC/MS Sensitivity, Reliability, Value Whether you need to quantitate drug metabolites, measure herbicide levels in foods, or determine contaminant levels in ground water, the triple quadrupole mass spectrometer has proven itself to be without equal for quantitating trace organic compounds in dirty matrices. The Agilent 6410 Triple Quadrupole LC/MS establishes a new standard for value in a triple quadrupole mass spectrometer; delivering outstanding sensitivity, great ease of use, and legendary Agilent reliability: all at a very attractive price. Features Femtogram-level sensitivity - All ion-path components are designed to maximize ion generation and transmission across a broad mass range-for low limits of detection regardless of application. Fast, high-throughput MS/MS - The high-frequency hexapole collision cell uses linear acceleration to provide very fast MS/MS experiments without cross-experiment memory effects. Sensitivity remains excellent even with 5-ms dwell times. Rugged, reliable performance - Agilent's patented orthogonal spray ion source and high-capacity counter-current drying gas maximize performance across the widest range of flow rates and mobile phases, keep the ion optics cleaner, and reduce background. Ion source choices maximize versatility - The best selection of ion sources in the LC/MS industry-including the revolutionary multimode sources with true simultaneous ESI and APCI-allow the 6410 to address a wide range of applications New software increases throughput - All-new software maximizes convenience and throughput with features like automated tuning, parameterless integration, dynamically linked data, Curve-Fit Assistant, batch review, color-coded outlier flagging, and customizable reporting. MassHunter LC-MS/MS Quantitative Analysis Software

Triple Quadrupole: MRM means “SIM” Parent Ions Enter Collision Cell - Collide with Ar Gas and Dissociates Sensitivity Selectivity Detector API Source SRM on a triple quad is more sensitive than full scan MS/MS in an ion trap because both Q1 and Q3 are parked on a single m/z ion. This means are nearly 100% duty cycle because both mass analyzers are dwelling on a single ion. In the trap we must do an accumulation, which takes time and translates into a loss of sensitivity. Q3 is Parked Passing Only Product Ions of a Single m/z to the Detector Energy Q1 is Parked Allowing Only Parent Ions of a Single m/z to Pass to the Collision Cell Ion Guide Transports +/- Ions to Q1 +/- Ions and Neutrals Formed in API Source MassHunter LC-MS/MS Quantitative Analysis Software

Pipeline software subsystem architecture Acquisition Deliverables: Acquisition deliverables organized as Sequence table Analysis File versioning of method, data, results can be handled by SSI’s XDrive. Deliverables: Reporting results including graphics and calculations Reporting Deliverables: Reporting subsystem formats results and imports graphics MassHunter LC-MS/MS Quantitative Analysis Software

Agilent 6410 QQQ Quantitative Analysis Software Sensitivity, Reliability, Value Whether you need to quantitate drug metabolites, measure herbicide levels in foods, or determine contaminant levels in ground water, the triple quadrupole mass spectrometer has proven itself to be without equal for quantitating trace organic compounds in dirty matrices. The Agilent 6410 Triple Quadrupole LC/MS establishes a new standard for value in a triple quadrupole mass spectrometer; delivering outstanding sensitivity, great ease of use, and legendary Agilent reliability: all at a very attractive price. Features Femtogram-level sensitivity - All ion-path components are designed to maximize ion generation and transmission across a broad mass range-for low limits of detection regardless of application. Fast, high-throughput MS/MS - The high-frequency hexapole collision cell uses linear acceleration to provide very fast MS/MS experiments without cross-experiment memory effects. Sensitivity remains excellent even with 5-ms dwell times. Rugged, reliable performance - Agilent's patented orthogonal spray ion source and high-capacity counter-current drying gas maximize performance across the widest range of flow rates and mobile phases, keep the ion optics cleaner, and reduce background. Ion source choices maximize versatility - The best selection of ion sources in the LC/MS industry-including the revolutionary multimode sources with true simultaneous ESI and APCI-allow the 6410 to address a wide range of applications New software increases throughput - All-new software maximizes convenience and throughput with features like automated tuning, parameterless integration, dynamically linked data, Curve-Fit Assistant, batch review, color-coded outlier flagging, and customizable reporting. MassHunter LC-MS/MS Quantitative Analysis Software

MassHunter Quantitative Analysis Software Video Organization Batch-at-a-Glance 1. Powerful sample and compound data visualization capabilities Method Editor Sensitivity, Reliability, Value Whether you need to quantitate drug metabolites, measure herbicide levels in foods, or determine contaminant levels in ground water, the triple quadrupole mass spectrometer has proven itself to be without equal for quantitating trace organic compounds in dirty matrices. The Agilent 6410 Triple Quadrupole LC/MS establishes a new standard for value in a triple quadrupole mass spectrometer; delivering outstanding sensitivity, great ease of use, and legendary Agilent reliability: all at a very attractive price. Features Femtogram-level sensitivity - All ion-path components are designed to maximize ion generation and transmission across a broad mass range-for low limits of detection regardless of application. Fast, high-throughput MS/MS - The high-frequency hexapole collision cell uses linear acceleration to provide very fast MS/MS experiments without cross-experiment memory effects. Sensitivity remains excellent even with 5-ms dwell times. Rugged, reliable performance - Agilent's patented orthogonal spray ion source and high-capacity counter-current drying gas maximize performance across the widest range of flow rates and mobile phases, keep the ion optics cleaner, and reduce background. Ion source choices maximize versatility - The best selection of ion sources in the LC/MS industry-including the revolutionary multimode sources with true simultaneous ESI and APCI-allow the 6410 to address a wide range of applications New software increases throughput - All-new software maximizes convenience and throughput with features like automated tuning, parameterless integration, dynamically linked data, Curve-Fit Assistant, batch review, color-coded outlier flagging, and customizable reporting. 2. Create and edit an MRM method in a simple stepwise scenario Excel-base Reporting 3. Flexible and customizable solution based on XML-mapping MassHunter LC-MS/MS Quantitative Analysis Software

Agilent 6410 Triple Quad LC/MS Software Mass Hunter QQQ Quantitative Analysis Software The videos involve quite a bit of demos and not much slide whipping. I’ve tried to mention why we designed the software in a particular manner So that you can gain a deeper understanding – And thus put the features to work in your environment. As always, thank you for your attention. Agilent Technologies MassHunter LC-MS/MS Quantitative Analysis Software