0 Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS) New Lorne Bore (RN 17263). Depth 1613 m, Temp: 89 o C, pH 8.5); Flow rate: 7.56 L/sec; Chemistry: typical Na-HCO 3 -Cl Brisbane Sampling at bore source Sampling below bore source
1 Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS) The New Lorne Bore (RN 17263) runoff channel Temperature specific microbial colonisation occurs in the New Lorne bore run off channel in a temp gradient (88 o C to ambient) Sampling microbial mats
2 Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS) Grey Filaments at 75ºC Red Filaments at 66ºC Brown Filaments at 52ºC Green Filaments at 57ºC 4 spatially distinct colored microbial mats colonise New Lorne bore runoff channel at different temperatures
5 Samples (4 mats + source sample) HMW DNA extracted 16S rRNA genes cloned 400 clones studied to determine phylogroups Representative of each phylogroup sequenced Phylogenetic Reconstruction
D ictyoglomus Thermodesulfobacteria Bacteroides Thermomicrobia Aquifex Chrysiogenetes Thermotoga T hermales Firmicutes Chlamydia Deferribacter Nitrospir a Cyanobacteria Actinobacteria V errucomicrobia Acidobacteria Planctomycetes Fibrobacter Spirochetes Fusiforms Proteobacteria Microbial diversity of the New Lorne bore & the 4 runoff channel microbial mats (10 / 21 kingdoms)
5 Microbial Gene Research & Resources Facility (MGRRF) Institute for Molecular & Cellular Therapies (IMCT) Biomolecular & Biomedical Sciences (BBS) The microbial diversity profile of the 4 microbial mats
Quantitative diversity based on clones (in %)