Career Seminar Series IVF Embryologist Dr Gayle M. Jones Senior Research Fellow Monash Immunology & Stem Cell Laboratories

Tertiary Training B.Sc.(HONS) University of Melbourne Ph.D. Department of Pathology, University of Melbourne Certificate Recombinant DNA Techniques – Micromon, Monash University

Career Path NH&MRC Senior Technical Officer – Royal Women’s Hospital NH&MRC Research Officer – Royal Women’s Hospital IVF Embryologist – Royal Women’s Hospital Visiting Research Fellow – UC Irvine, California Director of Embryology – Melbourne IVF Research Fellow – Monash Institute of Reproduction & Development Senior Research Fellow – Monash Institute of Reproduction & Development Senior Research Fellow – Monash Immunology & Stem Cell Laboratories

Career Opportunities for Scientists in Assisted Reproduction Clinical Embryologist/Preimplantation Geneticist Laboratory Scientist – Pathology Andrology Endocrinology Research Scientist Human Animal species

Day 0 – Oocyte Retrieval

Day 0 - Insemination

Intra cytoplasmic sperm injection - ICSI

Day 1 – Fertilization check

Embryo Culture – Day2-Day5/6

Embryo Transfer

EMBRYO BIOPSY 1st PB 2nd PB ICSI 1-2 / 8 cells Trophectoderm  20 cells Day 0 Day 1 Day 3 Day 5

Hole in the zona pellucida by non-contact laser Insertion of glass micropipette Removal of a single blastomere for genetic analysis Embryo Biopsy – Day 3 Embryo

XXXYXXX Fluorescence In Situ Hybridization XX XY XXX XO Monosomy 21 Trisomy 21

Results of Clinical PCR for Cystic Fibrosis – Day 3 Embryos Father (carrier) Embryo (carrier) Embryo (affected) Embryo (unaffected) Mother (carrier) Delta F508Normal