Lecture 8 NMR Spectroscopy Basics and applications in biology
Lecture overview Basic principles of NMR spectroscopy NMR of small molecules NMR of proteins
NMR needs high magnetic fields A good introduction into the basic principles of NMR: http://web.mit.edu/speclab/www/PDF/DCIF-IntroNMRpart1-theory-o07.pdf For YouTube fans: http://www.youtube.com/watch?v=uUM5BNBULwc
NMR = nuclear magnetic resonance 1H, 13C and 15N nuclei have a very small magnetic moment: “spin 1/2” For a single spin: two energy levels in a magnetic field 2pn = -gB0 n: frequency, g: a constant, B0: external magnetic field E: energy A spectrum always shows peaks as a function of frequency
More than a single spin Chemical shifts Measured in ppm (“parts per million”) relative to a reference Different chemical environments cause different chemical shifts 1.2 ppm 3.6 ppm D
More than a single spin Scalar coupling constants Measured in Hz (“Hertz”, s-1) Caused by different spin states of neighboring spins (“parallel” or “antiparallel” to B0) Between spins separated by 1, 2 or 3 chemical bonds Doublet: 1 coupling partner Triplet: 2 coupling partners Quartet: 3 coupling partners D
NMR of urine: metabolomics Lots of compounds detected simultaneously (“multiplexing”) Peak integrals are directly proportional to abundance From: Wang Y et al. PNAS 2008;105:6127-6132
2D NMR Two frequency axes (ppm) Often symmetrical about the diagonal Correlates peaks in 1D NMR spectra (plotted on the sides) Diagonal peaks Same as 1D NMR spectrum Cross-peaks Connect different peaks in 1D NMR spectrum Arise from scalar couplings or other magnetisation transfer mechanisms From: http://www.chem.queensu.ca/facilities/nmr/nmr/webcourse/cosy.htm
Metabolomics 13C-1H correlation - Greatly improved spectral resolution H2O 13C 1H From: http://genomics.uni-regensburg.de/site/gronwald-group/research/metabolomics-by-multidimensional-nmr
Protein NMR H2O ppm
Folded versus unfolded protein Different chemical environments cause different chemical shifts
2D NMR of proteins - HSQC 15N-HSQC spectrum Correlates 15N and 1H NMR spectra Magnetisation transfer by the scalar coupling between amide nitrogen (15N) and amide proton (1H) Only cross-peaks, no diagonal peaks 15N 1H
2D NMR of proteins - HSQC 15N-HSQC spectrum One peak per backbone amide Two peaks per side-chain amide C O 15N 1H C O Cα 15N 1H R H
2D NMR of proteins - HSQC HSQC = ‘heteronuclear single-quantum coherence’ Higher magnetic field B0 improves resolution and sensitivity Protein must be enriched with 15N Grow E. coli on medium with 15NH4-salt as only nitrogen source Natural abundance of 15N: 0.3% 500 MHz 950 MHz
Resonance assignment Resonance assignment = attribution of a peak in the NMR spectrum to the specific nucleus in the molecule it comes from Needs a combination of NMR techniques 2D NOESY (NOE spectroscopy) is most important NOESY cross-peaks arise from nuclear Overhauser effects (NOEs) between 1H spins NOEs arise from through-space dipolar interactions provide a mechanism for magnetisation transfer NOE intensity proportional to 1/r6 (r = internuclear distance) observable for spins closer than ~5 Å A NOESY cross-peak shows that two 1H spins are in close proximity
NOESY example NOESY Symmetrical about diagonal Diagonal peaks correspond to 1D NMR spectrum chentobiose
Protein NMR spectra NOESY In principle sufficient information to calculate the 3D structure of the protein
3D NMR spectra For proteins enriched with 15N and 13C
A bit of history Nobel prizes for NMR spectroscopy 1952 1991 2002 Felix Bloch Edward Purcell Richard Ernst Kurt Wüthrich Chemistry: 3D protein structures by NMR Physics: discovery of NMR Chemistry: FT-NMR, 2D NMR
and more… 2003 Peter Mansfield Paul Lauterbur Medicine: MR imaging
3D structures of proteins by NMR Each NOESY cross-peak presents a distance restraint NOESY spectrum 3D structure
3D structures are defined by dihedral angles Amide bonds are planar The backbone conformation of each amino acid residue is defined by a f and a y angle Bond lengths and bond angles are known -> 2 degrees of freedom per amino acid backbone
Scalar couplings reflect dihedral angles Karplus curve 3-bond couplings (1H-C-C-1H) depend on the dihedral angle a Can be measured also for 1H-N-C-1H (backbone dihedral angle f)
NMR structures The NMR structure of a protein is presented as a bundle of conformers Each conformer presents a good solution to the NMR restraints First conformer usually is the best structure Typically a bundle of 20 conformers is deposited in the PDB
Mobility NMR works in solution Can measure conformational exchange Different experiments for different time scales
Drug development NMR is sensitive to changes in chemical environment Ligand binding changes the chemical shifts Sensitive also to weak binding Gold standard for site-specific ligand binding Large chem. shift changes induced by compounds 1 and 2 are highlighted in different colours Science 1996, 274, 1531-1534
Summary I NMR owes its success to Long life of the excited magnetisation (seconds) Low energy (400-1000 MHz = radiofrequency) Only nuclear spins in a magnetic field can absorb such small energy quanta High abundance of 1H (99.985%) Sensitivity to the chemical environment Drawbacks of NMR Relatively low sensitivity Expensive magnets Hard to become an expert
Summary II NMR spectroscopy is the most versatile spectroscopy on earth Multidimensional Most powerful analytical tool for chemists Metabolomics 3D structures of proteins Mobility information Ligand binding MRI NOT radioactive
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