Reaqua BT99

1.Reaqua BT99 Reaqua BT99 is a betaine,betaine is discovered by the Scheibler, and separated from the sugar beet, It is a completely natural, edible amino acids. Since 1939, DU Vigneaud found its nutritional function,it has become a hot topic of research, and is widely used in the pharmaceutical, food, additives, chemical reagents, chemical raw materials, livestock breeding, aquaculture and other fields.

Chemical structure： The Physical properties of Reaqua BT99 ❀White crystalline powder ❀Sweet and bitter taste ❀Soluble in water, ethanol, PG and glycerin ❀Solubility: 160g Reaqua BT99 / 100g water 8,7g Reaqua BT99 / 100g ethanol slightly soluble in ether ❀Compatible with anionic, cationic and nonionic materials ❀High temperature resistant ❀Heated to 310°decomposition ❀Loss on drying(105℃, 2 hours) ≤1.0% ❀Reaqua BT99 Content ≥99.0% ❀Residue on ignition ≤0.1% ❀PH Value (5%) 5.0-7.0 ❀Sulphate ≤20 ppm ❀Heavy metal(Pb) < 10 ppm ❀Arsenic < 2 ppm The Physical properties of Reaqua BT99

2.The function of Reaqua BT99 2.1 Reaqua BT99 is a major osmolyte K+ Sugars Amino Acids Reaqua BT99 H2O Water follows osmotically active compounds such as potassium and organic osmolytes Water doesn`t move alone

Disturbance Original cell volume Swell Shrink Recovery Osmolyte efflux Osmolyte uptake Cells regulate their volume and water balance by absorbing or effluxing osmolytes

2.2 Maintain moisture balance Water is common base to all biological solutions and thus to life itself All cells are 85-95% water Reaqua BT99 attracts water but does not immobilize it as a moisturizer

2.3 Solubilization of insoluble material, such as salicylic acid and allantoin Use levels from 2 to 10 %. Solubility in water is 0.2 % at room temperature Reaqua BT99 was found to increase the solubility of salicylic acid to 5 % Allantoin -solubility in water 0.7 % at RT -Reaqua BT99 was found to increase the solubility of allantoin to 2 %

2.4 Buffering properties of Reaqua BT99 pH values after addition of HCl 1N to 100 mls of 1M Reaqua BT99 aqueous solution

2.5 Polymers swelling Viscosity values during the swelling phase of 1% water and water- Reaqua BT99 dispersions of Sodium carboxymethylcellulose 0,14% Na CARBOMER aq

2.6 Reaqua BT99 ability to reduce the irritating effects of detergents Human patch test SLES/Reaqua BT99 on 25 volunteers 1. Test solution: 10.3 % SLES 0 - 7 % Reaqua BT99 2. 25 µL of the test solution was applied on the forearm in a „Finn chamber“ (sealed Al-chamber) 3. Removal of the Finn chamber after 24 and 48h, respectively. 4. Dermatologists assessed skin irritation 1 hour after removal

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Inositol, & Taurine uptakes 2.7 Osmotic Stress : UV radiation Oxidative Stress Opening of K+ channels Cell shrinkage Increased Reaqua BT99, Inositol and Taurine transporters & uptake Increased Reaqua BT99, Inositol, & Taurine uptakes (Warskulat et al. 2004) Inositol Reaqua BT99 Taurine

2.8 Sensory properties Reaqua BT99 modifies the feel of aq. Solutions and emulsions with 3-4 % ❀Glycerol ❀Sorbitol ❀Propylene glycol Best performer ❀50/50 glycerol/ Reaqua BT99 3-4 %

2.9 Improving the performance of foam Foam texture when 0% Reaqua BT99 included Foam texture when 5% Reaqua BT99 included

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