SPERM HY-LITERTM SPERM HY-LITER PLUSTM

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Presentation transcript:

SPERM HY-LITERTM SPERM HY-LITER PLUSTM Introduction to SPERM HY-LITERTM Tests for Forensic Laboratories Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual Assault Evidence SPERM HY-LITERTM SPERM HY-LITER PLUSTM

SPERM HY-LITERTM Test Format: Microscopic Screening Method for Sperm Antigen Detected: Human Sperm Heads Antibodies: Mouse monoclonal Labeling: Sperm: Alexa 488 / Nuclei: DAPI Sensitivity: Single Sperm Head Cross-reaction: None detected Test Read-out: Visual Preparation Time: 120 minutes – 10 minutes ‘hands-on’

Sensitivity & Specificity Buccal, Urine & Blood Extracts SPERM HY LITER - SPERM HY-LITERTM Phase Contrast SPERM HY-LITERTM staining Sensitivity & Specificity Buccal, Urine & Blood Extracts Down to a Single Sperm DAPI Filter FITC Filter

Animal Semen (various) SPERM HY LITER - SPERM HY-LITER PLUSTM Phase Contrast SPERM HY-LITERTM staining Species Specificity Animal Semen (various) without Human Semen DAPI Filter FITC Filter

Animal Semen (various) SPERM HY LITER - SPERM HY-LITERTM Phase Contrast SPERM HY-LITERTM staining Species Specificity Animal Semen (various) with Human Semen DAPI Filter FITC Filter

SPERM HY-LITERTM SPERM HY-LITER PLUSTM First Positive Identification for Human Sperm First Specific Identification for Human Sperm Sensitivity down to a single sperm Fully Compatible with Automated Methods Scan slides at lower magnification: 40X

Acknowledgements: SPERM HY-LITERTM SPERM HY-LITER PLUSTM Jennifer Old, Ph.D. Brett Schweers, Ph.D. P.W. Boonlayangoor, Ph.D. Chicago Liz Graffy, MS Marisa Farhner, M.S

Questions Comments Samples Gripes Karl Reich Tel 708.234.1200 Fax 708.978.5115 karl@IFI-test.com