If its Sour, If its slippery Carolina #212020 $61.25.

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Presentation transcript:

If its Sour, If its slippery Carolina # $61.25

Objective This lab is intended help student understand the properties of acids, bases and buffer solutions, while using evidence from a crime scene to solve the case.

Materials supplied 1.microplates 2. pH paper 3.(1) bottle, pH7 4. (2) bottles sodium hydroxide &hydrochloric acid 5. (10) blank label 6. 1 BC powder 7. (1) bottles NaCl, 20g & ssodium bicarbonate 8. (1) can of tomato juice 9. (3) microspoons, 1g

A. Procedure 1. Place a microspoon full of each solid unknown in a separate microplate add 1 mL of water to each solid. *NOTE: Label cabbage juice as purple liquid & pH7 buffer as Colorless solution. 2. Place 1 mL of each unknown liquid into separate wells. Record the placement. 3. Measure the pH of each unknown substance.

B. Procedure 4. Place 1 mL of purple liquid in the two microplate wells. To one of the wells, add 0.1M HCL dropwise until there is a significant color change. 5. To the second purple liquid well add 0.1M sodium hydroxide, counting the drops until there is significant color change. *How many drops does it take to bring about a significant color change? What are the pH of the purple liquide? 6.Place 1mL of the purple liquid in a microwell. Add 1 mL of the colorless solution and mix. Add dropwise counting the drops to the mixture gently shke the plate to mix

C. Procedure 7. Repeat step6 adding sodium hydroxide

Prior information Define acids & bases

Changes to the lab 1. Inform students how many drops to add to do the color changes. 2. Either inform the student of the substance or label the liquids as unknown to create a mystery. 3. Specify which wells the liquids are placed. Require students to label their lab sheets with the name of each substances also indicating the pH.