Biotest Medizintechnik GmbH Hemoglobin determination Biotest Hemoglobin System Accuracy and precision Biotest Medizintechnik GmbH

Products Cuvette Measuring device Biotest Hemoglobin Biotest Hemoglobin Tester

Function Cuvette Biotest Hemoglobin Tester Blood capillary, reagent and measuring cuvette in one. Biotest Hemoglobin Tester Photometer with integrated control and digital display

Function - Cuvette Chemical reaction Haemolysis by sodium desoxycholat Sodium nitrite Sodium azid Methemo-globin Free Haemoglobin Azid- Methaemo-globin Erythrocytes

Function - Cuvette Absorbance curve

Measurements

Measurements Linearity and precision, n = 20 measurements by each blood sample. y = 1,0064x - 0,0773 R² = 0,9992 5,00 10,00 15,00 20,00 5,0 g/dl 10,0 g/dl 15,0 g/dl 20,0 g/dl Biotest Hemoglobin System, g/dl Mean value HiCN, i g/dl

Measurements

Function - Tester Photometer; two wave lengths Evaluation and calculation Reading Evaluation

Self calibration 100% green light, 100% red light and dark current Compared to moment of calibration If necessary automatic adjustment of LED current Evaluation

Resolution Max deviation = +/- 0,75 % or +/- 30 steps Absorption 256 g/L 0 = 100 % light 470 – 880 nm 12 bits = 4000 ”steps” Resolution

Calibration Absorption 256 g/L Two points of calibration for linearity; 80 and 220 g/L Three control levels for linearity 120, 150, 180 g/L 0 = 100 % light 470 – 880 nm 12 bits = 4000 ”steps”

Function - Tester Calculation of Hb Extinction Turbidity Turbidity 500 570 600 700 800 880 Wave length [nm]

Capillary blood Hb Time 0 min 1 min 2 min 3 min

Results - Introduction Accuracy How close to true value is the result Precision How close to each other are repeated measurements with the same sample

Results - Introduction Accuracy and precision High accuracy Low precision Low accuracy High precision High accuracy High precision

Results - Introduction Accuracy: Deviation (mean) in g/L Preferred; deviation in % Precision: Standard deviation SD Preferred; Coefficient of variation, CV %

Results Precision with control blood (n=50) Mean value 12,39 g/dl 30 Mean value 12,39 g/dl SD 0,084 g/dl CV 0,68 % 25 20 Numbers 15 10 5 12,1 12,2 12,3 12,4 12,5 12,6 12,7 Result [g/dl]

Features Cuvette Aimed for Hb-measurement with Biotest-Hemoglobin-Tester and compatible instruments High accuracy and precision Can be used for venous, capillary and arterial blood Developed to be price worth due to latest production and control technique

Features Tester Automatic calibration: no calibration or test cuvette needed Cuvette holder minimizing the risk of contamination Automatic monitoring of power supply with fast loading of batteries (1 hour loading > 40 hours of measuring) Automatic shut off with latest value saved Measures only cuvettes with properly haemolysed blood

Summary High accuracy and precision Easy and reliable system Long time use on battery power

Results - Introduction