From the Box to Packing in 30 minutes

Pack-in-a-Box components Remove components and assemble bracket as shown Follow the instructions to pack your first column and learn how to use the system

Additional Equipment & Chemicals ¼ wrench 5/8 wrench 7/16 wrench Phillips head screwdriver 30ml beaker Waste container Methanol Chloroform Spatula Balance Computer Ultrasonic bath

Pre assembled for supplied SSI column hardware. Column Packer Assembly

Mounting Packing Stand to Pump Screw stand to side of pump using the two #6 screws provided.

Plumbing the Pump

Priming pumps Use MeOH for this column Open prime purge valve press prime button With prime purge valve open use the supplied syringe to draw solvent through the pump Prime pump When pump is primed close the prime purge valve

Packing Ring Seal Installation Place ring in adapter as shown Make sure ring is flat in bottom of adapter

Packer Mounted to Stand Mount packer to stand and tighten wing nuts No wrenches are required to seal reservoir Hand tighten end nuts

Column Assembly Assemble lower fitting of column and add frit to top end fitting and set aside to put on column after it is packed.

System Assembled Correctly The use of the Quick-Set software is not required but gives the most flexibility and will be used for this example

Installing Quick Set Insert Disk Run the Quick-Set setup.exe program file & follow prompts. Due to the many versions of Windows in use, your PC may have newer files than the ones being loaded. You will be prompted for a course of action, when in doubt,keep your existing files orignore other messages. Connect RS 232 Cable provided to the computer and pump Turn on pump

Click QuickSet Go to Start/Programs Click on Quick-Set The screen below appears with the information on the program

Automatic Set Up Initial screen is for locating the SSI / LA pump If communication does not initialize or if the pump is not on this list: Click on the Poll Com Port For Device button. This will scan 9 possible PC COM ports for the attached device. When the device is found, click on the Continue button.

Getting To CP Screen

Setting Flow and Pressure Set to pressure limited ramp and click send Set upper pressure to 9999 psi; click send Set lower pressure to 0 psi; click send Set ramp speed to 100; click send Set max flow and pressure from table

Adding Steps

Packing program to load PressureFlow RateTime Send to pump

Saving Your Packing Program Click on Save Settings Enter file name Save

Mounting Column Make sure packing ring seal is in adapter Hand tighten column in adapter Place plug in bottom of column Remove plug just prior to starting pump

Tighten Column on Packer Use a ¼ wrench and 5/8 or adjustable wrench to snug column to packer Do not over tighten ¼ turn past snug is fine Fill column with 3 mL MeOH

Setting up Graphing and Data Collection

Saving Packing Data Select Log data Give file a name

Making the Slurry Weigh 3 gm of silica into 30 mL beaker Add 20 ml of 50:50 Chloroform:Methanol (Straight MeOH can be used but is not as efficient) Use a spatula to break up packing Place in ultrasonic bath until uniformly dispersed Pour in packing reservoir as soon as possible and pack column

Adding Slurry Use 20 mL of silica slurry and pour carefully into reservoir Fill reservoir with washings of beaker and close

Is It Closed? Make sure the reservoir is full with slurry Remove silica from threads Hand tighten top nut

Make Sure You Are Ready Is the program loaded? Is the pump primed? Is there a waste collection vessel below the column? Did you tighten the fittings? Is there slurry in the reservoir and is the reservoir closed? Are you set to graph the process and save your data? If so, proceed

Packing The Column To run program, click on Pump; or if program is loaded click on Start

Initial Packing Profile

Running a Column Packing Profile

Removing the Column Stop the pump and let the pressure drop to 0 psi Carefully unscrew the column, and using a flat spatula or razor blade, remove excess silica Remove silica from threads if present Place end fitting and frit on column and seal

Removing Excess Silica Work quickly with a spatula edge or razor blade

Getting Ready to Pack Again Pump remaining silica from precolumn for reuse or disposal Empty reservoir Install next column and repeat steps