Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Signalment Female,10 year old Yellow-naped Amazon parrot

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only History 10 day history of anemia, lymphocytic leukocytosis and lethargy

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Physical exam No abnormalities noted initially

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Serial Chemistry Results Hypoproteinemia Hypoalbuminemia Hypophosphatemia Hypocholesterolemia Monoclonal gammopathy (Beta region)

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Diagnostic Work up Imaging Contrast radiography Contrast CT PETscan

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only

Histopathology Expansion and infiltration Round cell population Abundant cytoplasm Round vesicular nuclei Prominent multiple nucleoli Rare mitoses Anisokaryosis and anisocytosis Little intra-epithelial involvement

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Special Stains ZN and Fites acid fast stains were negative Considered cell size and plasmacytoid differentiation as indication of plasma cell tumor or lymphosarcoma Recommended immunohistochemistry

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Monoclonal CD3 Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only

Monoclonal CD3 Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only

IMHC Results CD79 did not produce staining in the avian splenic control or the intestinal infiltrate BLA.36 did not produce staining in the avian splenic control CD3 polyclonal and CD3 monoclonal stained splenic control tissue and the neoplastic cells

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Diffuse Intestinal T cell Lymphosarcoma

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Outcome Owner elected euthanasia after seven weeks of chemotherapy

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only

Discussion LSA is rare in birds but typically presents as cutaneous or peri-orbital lesions Not thought to be virally induced in psittacines

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Discussion No previous reports of diffuse T cell intestinal lymphosarcoma in psittacines Prognosis is poor despite chemotherapy as with the T cell immunophenotype in mammals. Unique findings in this case included gammopathy and lymphocytic leukosis

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Monoclonal Gammopathy (Beta) Might have more strongly supported B cell origin Been reported twice before with psittacine T cell lymphosarcoma Unlikely to result from unidentified infectious etiology

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only References Ramos-Vara JA, Smith EJ, Watson GL. Lymphosarcoma with plasmacytoid differentiation in a Scarlet macaw. Avian Dis 1997, 41: de Wit M, Schoemaker NJ, Kik MJL, Westerhof I. Hypercalcemia in two amazon parrots with malignant lymphoma. Avian Dis 2003, 47:

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Acknowledgements Dale Godson – Prairie Diagnostics Histopathology Laboratory - UT Ladonna Mrkonjich Dr. Donnell Dr. Roslyn Casimir

Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only Questions??