Murder at Notre Dame: The Football Stadium Narrated by Mr. Azar

The Low Down Nevin Longenecker was murdered on the football field during the run through for his honorary doctorates from Notre Dame. This gruesome murder is being investigated by 3 teams. These teams are the foremost experts in the field of DNA fingerprinting. YOU are a member of one of these teams!

DNA Fingerprinting This technique is relatively new to the field of Biology. The concept behind the procedure is looking at specific sequences of DNA base pairs between a sample DNA segment and possible suspects DNA samples. Normally the entire DNA sequence is started with and then cut with restriction enzymes to isolate specific sequences to compare.

Suspects There are 3 suspects who have been identified for this particular murder: Suspect 1: Dr. V Suspect 2: Charlie Weiss Suspect 3: Mike Brey

Forensic Procedures 1)Construct DNA – each member of team is responsible for a specific persons sample. 2)Use Restriction Enzymes – cut DNA at sites identified by NoRoI and NoRoII. 3)Use Gel Electrophoresis – use template gel and separate DNA segments by base pair size.

Forensic Procedures 4)Denature DNA – Separate double strands into single strands. 5)Radioactive Probes – Create probes and identify place on DNA strands. 6)Autoradiography – Record the location of the radioactive probes. 7)WHO DID IT??

Discussion Ideas and thoughts.... Demo model No H bonds no 5 3 Build only 1 strand Different dna for each table Mark/identify the 3 5 bead to avoid flipping Transcription or translation Green should be guanine Sticky notes, as replacing the beads