In The Name Of God Precipitation

Antigen/Antibody Reaction Primary reaction : Immunoassay :Like Elisa , RIA , IFA Secondary reaction : Lattice formation like Agglutination , Hemagglutination Complement Fixation, Neutralization , Precipitation : soluble antibody reacts with soluble antigen Tertiary reaction : Detected by affect on tissues or cells.

Secondary Phenomenon

precipitation : proteins Several types: Protein precipitation SRID DID ImmunoElectherophoresis: Counter current IEP Rocket IEP

Single Radial Immuno diffusion or Mancini SRID: Single Radial Immuno diffusion or Mancini SRID plate preparation: (Agarose ِ+ BUFFER ( Ab (Anti IgG ) Punch Sampling Incubation in RT for 24h measuring of diameter making of curve Calculation of sample concentration

SRID Plate

Single Radial Immuno diffusion : In SRID : 34 GAM = Is useful for measuring of IgG , IgA, IgM,C3, C4 Gel is mixed with Ab Ag is in punch In Single Radial Diffusion: one component is fixed (e.g. antigen-in this case it is IgG) As the Ag diffuses into the gel it reacts with the Ab and when the equivalence point is reached a ring of precipitation is formed as illustrated in Figure The diameter of the ring is proportional to the concentration of Ag .

(Double Immuno Diffusion)= DID or Ouchterlony Immunodiffusion Qualitative 2 Diffusion Application in research laboratory Is not as a routin method now

Ouchterlony - Identity

Ouchterlony – Partial Identity

Ouchterlony Non-Identity

measuring the amount of light Passing Through the solution. Turbidimetery measuring the amount of light Passing Through the solution.

Measures SCATTERED light Nephelometery Measures SCATTERED light

Immunoelectrophoresis Counter current IEP : Qualitative Rocket IEP: Quantitative

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