Microscopy Cell Structure Mitosis A&P 1 - Lab #2 Microscopy Cell Structure Mitosis

Arm Stage Condenser Illuminator Base Objective Lens Ocular Lens Rotatable Head Arm Mechanical Stage Objective Lens Mechanical Stage Control Stage Course Adjustment Knob Condenser Fine Adjustment Knob Illuminator Base

Magnification Total magnification is achieved by multiplying the power of the ocular lens by the power of the objective lens Total Magnification = Ocular Lens X Objective Lens

Objective Lens Red Band = Low Power = 4 4(obj lens) X 10(ocular lens) = 40X Yellow Band = Medium Power = 10 10(obj lens) X 10(ocular lens) = 100X Blue Band = High Power = 100 40(obj lens) X 10(ocular lens) = 400X White Band = Oil Immersion

Follow the Rules! ALWAYS begin on LOW POWER Once you have the specimen in focus Move to medium power Refocus, then you can move to high power Once you move off low power, you can NEVER, NEVER, NEVER use the coarse adjustment

Orientation of Specimen

Specimen is flipped horizontally & vertically

Depth of Field

Preparing a Wet Mount Slowly Lower Cover Slip Cover Slip 45O

Air Bubbles - Try Again!

Types of Cells There are 2 types of cells: Prokaryotic Eukaryotic (pro- before) (eu- true) -kary = nucleus

Prokaryotic Cells

Prokaryotic Cells ALL bacteria are prokaryotic Prokaryotic cells -lack a membrane-bound nucleus. -genetic material is present in the nucleoid ALL prokaryotic cells are bacteria, and ALL bacteria are prokaryotic The only place you will find prokaryotic cells in is the KINGDOM BACTERIA AND ARCHEBACTERIA

Eukaryotic Cells

Epithelial - Cheek Cells

The Cell Cycle Interphase Mitosis Cytokinesis Prophase - prepare Metaphase - middle Anaphase - apart Telophase - two Cytokinesis cyto- = cell -kinesis = to move

The Cell Cycle

Interphase

Mitosis - Prophase

Mitosis - Metaphase

Mitosis - Metaphase

Mitosis - Anaphase

Mitosis - Anaphase

Mitosis - Telophase

Mitosis - Telophase/Cytokinesis

Practice Identifying the Stages of Mitosis Select the link below (you must be in “slide show” mode) If you are unable to view a slide show, you can go to Bookmarks on Ocean Cruiser. The link is found there. Mitosis