4 TH ANNUAL ADVISORY PANEL MEEETING 10 January 2013 San Diego, California

Outline of Presentation  Project Overview  Morning Session  Afternoon Session

Project Overview

RosBREED Mission Statement We will develop and apply marker-assisted breeding, based on improved knowledge of industry value and consumer preferences, to accelerate and increase the efficiency of rosaceous cultivar release and successful cultivar adoption 4 yrs $7.2M federal $7.2M matching Amy Iezzoni, Michigan State Univ. Cameron Peace, Wash. State Univ. This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture

RosBREED Will Bridge the Chasm Genomics Resources Marker- assisted breeding Genomics knowledge More efficient development of new cultivars

RosBREED’s Focus is on Fruit Quality

RosBREED Will Develop Tools Marker-assisted breeding in application For those traits that have value for producers, market intermediaries and consumers

RosBREED Will Use Improved Knowledge Courtesy David Byrne, Texas A&M Univ.) of industry values and consumer preferences to select target traits for Marker-Assisted Breeding Impacts: increase new cultivar adoption enlarge market potential

Supporting Breeding Decisions Which traits to target? Which parents to use? Which combinations to create? Which seedlings to progress? Which selections to trial? Which advanced selections to commercialize?

Which wild germplasm to use? Which traits to target? Which parents & combinations to create? Which seedlings to progress? Which selections to trial? Which advanced selections to commercialize? Supporting Breeding Decisions

Long-term outcomes Four year project outcomes Increased gain in fruit quality per breeding cycle due to improved parent selection and improved mean progeny value More rapid availability of new cultivars with genetically superior fruit quality Improved profitability and sustainability of US rosaceous fruit, nut, and floral crops with increased consumption and enjoyment Implementation of MAB by breeding programs RosBREED’s Target Outcomes

Project Assistants Audrey Sebolt Joan Schneider Extension Team Leader: Cholani Weebadde Genomics Team Leader: Dorrie Main Genotyping Team Leader: Nahla Bassil BIMS Team Leader: Gennaro Fazio Socio- Economics Team Leader: Chengyan Yue MAB Pipeline Team Leader Cameron Peace Pedigree- Based Analysis Team Leader: Eric van de Weg Breeding Team Leader Jim Luby Apple  Jim Luby  Susan Brown  Kate Evans Strawberry  Chad Finn  Jim Hancock  Tom Davis Peach  Ksenija Gasic  John Clark  Dave Byrne  Tom Gradziel Cherry  Amy Iezzoni  Nnadozie Oraguzie Executive Committee Nahla Bassil Michael Coe Gennaro Fazio Karina Gallardo Ksenija Gasic Jim Luby Dorrie Main Jim McFerson Cameron Peace Eric van de Weg Cholani Weebadde Chengyan Yue Project Director Amy Iezzoni RosBREED’s Management

Demonstration Breeders & Breeding Trainees

Cook Harrison van Sickle Hirst Watkins Karp Abbott Buell Gardiner Hummer Cheng Simon Ross Industry Extension Scientific Allen Goldberger CarrOlmstead DoddSherman GrimArus KorsonAvari Lott Bierlink Curtis Perry Sparks Seavert Bliss Baugher Stokes Moffitt Advisory Panel Members

Plant Research International, Netherlands East Malling Research, UK CRA-FRU, Rome, Italy IASMA, Trento, Italy INRA ( Bordeaux, Avignon, Angers ), France Andres Bello University, Chile Plant & Food Research, New Zealand ARC, South Africa RosBREED participant location International Collaborators + 2 Scientific Advisory Panel members (Spain, New Zealand)

Morning Session Goal = provide information to AP members to set the stage for receiving advice

StartEnd Audience/ Group Presenter/moderatorTitle 8:008:30AllAmy IezzoniIntroductions 8:309:00AllAmy IezzoniOverview of impact 9:0010:00All Karina Gallardo Dorrie Main Nahla Bassil Eric van de Weg Gennaro Fazio, Jim Luby Target traits, Genome Scans, Pedigree-based breeding analysis, Toolbox and breeding (Obj. 1-3): Goals, Deliverables, Challenges, Year 4 Deliverables, and Q & A 10:0010:30 Break (BIM System computer demonstration available) 10:3011:00All Cameron Peace Cholani Weebadde MAB pipeline and extension (Obj. 4-5) Goals, Deliverables, Challenges, Year 4 Deliverables, and Q & A 11:0011:15AllMichael CoeEvaluation of Extension efforts 11:1511:45AllAmy/CameronPlans for RosBREED #2 11:451:00 Lunch Morning Session

Major Enabling Deliverables Enabling computer-based accomplishments that allow us to find, polish and put the Jewels into application Computer demonstrations at breaks: Eric van de Weg Pedigree-Based Analysis software Dorrie Main BIM System Cholani Weebadde Jewels in the Genome in eXtension

External Evaluation of Extension Efforts Dr. Michael Coe Cedar Lake Research Group, LLC

From Natural Diversity to Better Cultivars

Afternoon Session Goal = receive advice from AP members

1:001:30All Karina Gallardo Jim Luby Demo of apple auction experiment 1:003:00 Group 1 Discussion Session Group 2 Ideas and approaches for addressing industry opportunities, needs and limitations Ideas and approaches for addressing breeding opportunities, needs and limitations 3:003:30 Break (BIM System computer demonstration available) 3:304:15AllDS 1 & 2 moderatorsReports and Discussion – possible next steps 4:155:00AllCameron PeaceRound table 5:00AllDinner on your own or in working groups Afternoon Session StartEndAudience/ Group Presenter/ moderator Title Group 1: Remain in the ballroom Group 2: Move to Cortez room

Discussion Session 1.What methods or plans of work could be done differently to better meet RosBREED’s current industry and breeding project goals? 2.What unanticipated opportunities, needs and limitations should we address within the current project? 3.Thinking ahead to a second project, how well do our proposed objectives effectively address current and future industry and breeding opportunities, needs and limitations? What modifications would you recommend? 1:303:00 Discussion session GROUP 1: Remain in Ballroom Ideas and approaches for addressing industry opportunities, needs and limitations GROUP 2: Cortez Room Ideas and approaches for addressing breeding opportunities, needs and limitations 3:003:30 Break (BIMS computer demonstration available) 3:304:15AllDS 1 & 2 moderatorsReports and Discussion – possible next steps

Jewels in the Genome Analogy Find An individual’s genome is the full complement of genetic information that it inherited from its parents. Within this vast repertoire of genetic information, individuals genes are being discovered that control critical production and fruit quality traits. Put into application in breeding Jewels = valuable genetic discoveries ready for breeding application = marker-locus-trait associations validated in a breeding program Polish

RosBREED seeks to find, polish and put into application predictive DNA tests for valuable Jewels in the Genome

“Jewels in the Genome” Available Now!

The Jewels are Linked on Chromosomes 56 chromosomes in strawberry!!

Acknowledgements This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture

MSU Amy Iezzoni (PD) Jim Hancock Dechun Wang Cholani Weebadde WSU Cameron Peace Dorrie Main Kate Evans Karina Gallardo Vicki McCracken Nnadozie Oraguzie Former WSU Raymond Jussaume Mykel Taylor Cornell Susan Brown Kenong Xu Clemson Ksenija Gasic Gregory Reighard Texas A&M Dave Byrne Univ. of CA-Davis Tom Gradziel Carlos Crisosto Univ. of New Hamp. Tom Davis Univ. of Minnesota Jim Luby Chengyan Yue Oregon State Univ. Alexandra Stone Plant Research Intl, Netherlands Eric van de Weg Marco Bink USDA-ARS Nahla Bassil Gennaro Fazio Chad Finn Univ. of Arkansas John Clark