AB Figure S1 DNA content (% of control) **. MCF7:PF +E2 1 MCF7:PF +E2 2 MCF7:PF +E2 3 MCF7:PF +4OHT 1 MCF7:PF +4OHT 2 MCF7:PF +4OHT 3 MCF7:PF +ICI 1 MCF7:PF.

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AB Figure S1 DNA content (% of control) **

MCF7:PF +E2 1 MCF7:PF +E2 2 MCF7:PF +E2 3 MCF7:PF +4OHT 1 MCF7:PF +4OHT 2 MCF7:PF +4OHT 3 MCF7:PF +ICI 1 MCF7:PF +ICI 2 MCF7:PF +ICI 3 MCF7:PF E2+ICI 1 MCF7:PF E2+ICI 2 MCF7:PF E2+ICI 3 MCF7:PF 4OHT+ICI 1 MCF7:PF 4OHT+ICI 2 MCF7:PF 4OHT+ICI 3 A Figure S2 E2 4OHT 538 genes (sorted by level of regulation) E 2 regulated 4-OHT regulated E 2 and 4-OHT regulated B

Relative IGFBP3 levels ** # Relative GPER levels ** A B Figure S3 C Relative FOXO1 levels ** *

* Relative FOXO1 levels ** Relative GPER levels Relative IGFBP3 levels * * ** AB C Figure S4

** Relative c-Myc levels ** Relative Cyclin D1 levels Relative IGF-1R levels ** * A B C Figure S5

Figure S6 A B Relative EHD2 levels * ** Relative FHL2 levels * * Relative EHD2 levels * C