DNA POLIMERASE By: Muhartri Sanjaya 20512046 Nishia W Meray 20511034.

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Presentation transcript:

DNA POLIMERASE By: Muhartri Sanjaya 20512046 Nishia W Meray 20511034

reaksi polimerisasi deoksiribonukleotida DNA POLIMERASE Replikasi DNA Reparasi DNA reaksi polimerisasi deoksiribonukleotida Rantai DNA

Tatanama DNA Transferase EC.2.7.7.7 Kelas Transferase Gugus fosfat

Klasifikasi E. Coli Pol I (Kelas A) E. Coli Pol II (Kelas B) E. Coli Pol III (Kelas C) Euryarchaeotic Pol II (Kelas D) Pol β Manusia (Kelas X) E. Coli UmuC/DinB (Kelas Y)

Peran DNA Polimerase Elongasi Proofreading Pemanjangan rantai nukleotida Mengenali kekeliruan dan memperbaiki

Struktur DNA Polimerase Tumb Finger Palm

Mekanisme Kerja

Residu Katalitik & Efektor Efektor yang mengandung ion logam Residu Katalitik aspartat 705 dan 882

Aplikasi dan Modifikasi DNA Pol I PCR E. Coli Thermus Aquaticus Bukan Termofilik