© 2012 O’Brien & Gere # obgPresentation Are there Microbes Lurking? June 12, 2012 | State University of NY Paul W. Tranchell CIH, CSP, RBP Project Manager

© 2012 O’Brien & Gere Follow Us:Watch Us:Connect with Us: Like Us: Are there Microbes Lurking? June 12, 2012 | State University of NY 2

© 2012 O’Brien & Gere Occupational Stressors: (i.e. biological agents)  Anticipation  Recognition  Evaluation  Control Industrial Hygiene

© 2012 O’Brien & Gere Definition Normal Locations Selected Suppliers Unusual & Ancillary Biohazards Biosafety Fundamentals Agenda 4

© 2012 O’Brien & Gere Biosafety  The application of knowledge, techniques and equipment to prevent personal, laboratory, and environmental exposure to potentially infectious agents or biohazards  Biosafety defines the containment conditions under which infectious agents can be safely manipulated  The objective of containment is to confine biohazards and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents  Medicine.net

© 2012 O’Brien & Gere Normal Locations  Bloodborne Pathogens  Microbiological Laboratories  Animal Laboratories  Molecular Biology Labs

© 2012 O’Brien & Gere Select Suppliers  American Type Culture Collection (ATCC) - Repository of a huge number of cell lines American Type Culture Collection (ATCC)  CABRI (Common Access to Biological Resources and Information) CABRI (Common Access to Biological Resources and Information)  Developmental Studies Hybridoma Bank - Monoclonal antibodies Developmental Studies Hybridoma Bank  DSMZ - The DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH = German Collection of Microorganisms and Cell Cultures) is a non-profit public domain general repository specializing in tumor cell lines. It holds the largest collection of human hematopoietic cell lines currently available worldwide. DSMZ  European Collection of Cell Cultures (ECACC) - ECACC "was established in 1984 as a cell culture collection to service the research community and provide an International Depository Authority recognised patent depository for Europe." European Collection of Cell Cultures (ECACC)  HyperCLDB - Hypertext resource derived from the Cell Line Data Base (CLDB) with descriptions of more than 4,000 cell lines HyperCLDB  National Cell Culture Center - A "national resource facility established by the National Institutes of Health (NIH) to provide customized, large scale, cell culture services for basic research laboratories." National Cell Culture Center

© 2012 O’Brien & Gere Unusual & Ancillary Hazards  Field Work  Lyme Disease  Insect Bites  Suborder Surpentes  Rodent Diseases ›Plague ›Hanta Virus  HVAC / Legionella  Bird Droppings (no bleach please)  Mold  Suspicious Packages / ”white” powder  Drum Skins

© 2012 O’Brien & Gere Chain of Infection 9 Biosafety Fundamentals Risk Assessment Reservoir of pathogen Portal of escape Portal of escape Transmission Route of entry / infectious dose Susceptible host Incubation period Practices / Equipment PPE Access control / Immunization Surveillance Facilities

© 2012 O’Brien & Gere Definitions Biosafety LevelRisk Guide NIH 1 well characterized agents not consistently known to cause disease in healthy adult humans of minimal potential hazard to laboratory personnel and the environment Agents that are not associated with disease in healthy adult humans. Includes a list of animal viral etiologic agents in common use. 2 agents of moderate potential hazard to personnel and the environment Agents that are associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available. 3 indigenous or exotic agents which may cause serious or potentially lethal disease as a result of exposure by the inhalation route Agents that are associated with serious or lethal human disease for which preventive or therapeutic interventions may be available 4 dangerous and exotic agents which pose a high individual risk of aerosol-transmitted laboratory infections and life-threatening disease Agents that are likely to cause serious or lethal human disease for which preventive or therapeutic interventions are not usually available 10 kcd11/HE.prn/NYCER-Biosafety/NYCER_BSL2& pptx Biosafety Fundamentals

© 2012 O’Brien & Gere Control Strategies  Standard Microbiological Practices  Special Microbiological Practices  Safety Equipment  Primary Barrier ›Biological Safety Cabinets (BSC) ›Types, Selection, Use!  Personal Protective Equipment  Laboratory Facilities  Secondary Barriers 11 kcd11/HE.prn/NYCER-Biosafety/NYCER_BSL2& pptx Biosafety Fundamentals

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