AGH University of Science and Technology

Slides:

Advertisements

Similar presentations

Simulation: An Investigative Tool in the Life Sciences & Engineering.

Advertisements

Label des Veranstalters Department of Sport Science and Kinesiology 1 Department of Sport Science and Kinesiology Name.

Sensitivity of Human Lymphocytes to Low-Dose Radiation

Cebulska-Wasilewska A. 1,2, Miszczyk J. 1, Dobrowolska B. 3, Dobrowolski Z. 3 1 Environmental and Radiation Biology Department, The H. Niewodniczański.

Wet Lab Radiation-Induced Chromosome Damage and Rejoining Background Background Equipment Equipment Supplies Supplies Procedures Procedures Troubleshooting.

Important Tools in Biology. 1. Centrifuge Used to separate the parts of the cell by spinning it around a solution and separating it into several layers.

Online Counseling Resource YCMOU ELearning Drive… School of Architecture, Science and Technology Yashwantrao Chavan Maharashtra Open University, Nashik.

Prof. Dr. E. A. Kamel Department of Biological Sciences and Geology Faculty of Education Faculty of Education Ain Shams University Ain Shams University.

Wet Lab Radiation Damage Measured by Micronucleus (MN) Assay Background Background Equipment Equipment Supplies Supplies Procedures Procedures Lab Demonstrations.

Host parasite interactions. Table 28-1 Table 28-2.

Chapter 6 Cytogenetics: Karyotypes and Chromosomal Aberrations

PowerPoint Presentation Materials to accompany

Studying of regularity of programmed cell death after influence of demages factors Speaker: Karolina Socha 2011 Student Practice in JINR Fields of Research.

IAEA International Atomic Energy Agency Premature Chromosome Condensation (PCC) Analysis Lecture Module 6.

The use of M-FISH to analysis the presence of genome instability in cells exposed to orthopaedic implant wear debris Presented by: Martin Figgitt Bristol.

IAEA International Atomic Energy Agency Cytogenetic Dose Estimation in the Criticality Accident in Tokaimura Lecture Module 13.

IAEA International Atomic Energy Agency Applied Statistics for Biological Dosimetry Part 2 Lecture Module 8.

Dr. R.A. Siddique M.V.Sc PhD Scholar National Dairy Research Institute Karnal, (Haryana) India.

IAEA International Atomic Energy Agency Basics of Biodosimetry Part 1 Lecture Module 2.

CENTRAL LABORATORY FOR RADIOLOGICAL PROTECTION IAEA PROJECT RER/9/117 IAEA PROJECT RER/9/117 Regional Workshop on Regulatory Control of Radioactive Discharges.

PRINCIPLES OF DETECTION OF RADIATION INJURES. Accidental dosimetry PHYSICAL DOSIMETR Y BIOLOGICAL DOSIMETRY CLINICAL DOSIMETRY DOSE RECONSTRUCTION, Personal.

 Most cultural heritage objects concern monuments and buildings, are mainly built by stone.  The ecological requirements of endolithic organisms as well.

Tools and Procedures Measurements. A Common Measurement System.

Measurement of Delayed-Neutron Yield from 237 Np Fission Induced by Thermal Neutrons Frank Laboratory of Neutron Physics - Dubna G.

IAEA International Atomic Energy Agency Automatic Analysis of Chromosomal Assays Lecture Module 9.

Applied Statistics for Biological Dosimetry Part 1

Chromosome Dropping The final cell suspension in 3:1 methanol:acetic acid fixative was then dropped from varying heights: 0 cm, 1 cm, and 30 cm. A ringstand.

Part No...., Module No....Lesson No. Biological Dosimetry Lecture

Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans H.Schröder, A.Heimers, R.Frentzel-Beyme, A.Schott and W.Hoffmann.

Cytogenetics B.Sc. MLT fourth semester. Chromosome Abnormalities A chromosome abnormality reflects an abnormality of chromosome number or structure. There.

Mitosis  Is a process of cell division which results in the production of two daughter cells from a single parent cell. The daughter cells are identical.

2010 Student Practice in JINR Fields of Research

Q1. Ten blood samples are set up, cultured by routine methods. All Showed low Mitotic Index, EXPLAIN? briefly!

Karyotyping – 1.

Fluorescence microscopy Principle and applications.

Title Katsumi Furitsu MD.Ph.D Board and science team member of ICBUW Brussels, May 15th, 2007 For the Ban of Uranium Weapons Scientific and Medical Grounds.

Remon Ibrahim Remon Ibrahim High Energy Group.  Introduction  Experimental Techniques  Results  Conclusions Content.

Dicentric Assay Lecture Module 4 Lecture: Dicentric assay

The Effect of Fluorescent Labelling on Deformability and Geometric Properties of Red Blood Cells Joanne Wong Supervisor: Dr. D. Jackson March 23,2011 Medical.

Microscope A microscope is a tool that helps scientists examine objects that are very, very small. Microscopes magnify objects that the human eye cannot.

Radiological Accidents in Georgia

B-1.2: Use appropriate laboratory apparatuses, technology, and techniques safely and accurately when conducting a scientific investigation.

This paper has been carried out with financial support from the Commission of the European Communities, specific RTD programme "Quality of Life and Management.

1 Radiobiology & Radiobiology of Accelerated Heavy Ions Haitham A. Shaban Haitham A. Shaban Assistant Researcher, NRC Teaching Assistant, American University.

Study Guide. Most common, specimen is darker than background Bright Field Used to see bacteria from syphilis Used for viewing live unstained specimens.

LABORATORY OF RADIATION BIOLOGY Radiaton Induced Apoptosis in Human Lymphocytes Joint Institute for Nuclear Research Karolina Ficenzova, Martin Sefl Faculty.

Home Lesson: Karyotypes Unit: Genetics. What is a karyotype?? A karyotype is a picture of a person's chromosomes. In order to get this picture, the chromosomes.

Cytokinesis Block Micronucleus (CBMN) Assay

Light Microscope.

Laboratory of Radiation Biology

Practical Of Genetics Human karyotype.

It’s Time to Science Lesson

and Rafi Korenstein1 ,Avi Gover2 Avital Korenstein-Ilan1

Estimating Radiation Exposure using Chromosomal Aberrations in Human Lymphocytes Team Members: Julie Asiello, Laura Banks, Leah Carmichael, Christopher.

Piotr Knapp Piotr Wróblewski

Laboratory of Information Technology

دانشگاه شهیدرجایی تهران

تعهدات مشتری در کنوانسیون بیع بین المللی

Figure 5 The biological effects of charged particles

Koch’s Investigation of Anthrax

Summer Students Practice,

Dicentric Assay Lecture Module 4 Lecture: Dicentric assay

Detectors in the FASA experiments

Carola Hartel, Elena Nasonova, Martina C. Fuss, Anna V

Human karyotype By: Mahmoud W. El-Hindi

Opposition 11. Grow light Opponent: Maria Krasnova Team of Belarus.

Problem Solving  5. What type of cells are shown above?

General Genetics Lab # 4&5 Human karyotype.

„HPGe detector technologies”

Scientific Tools and Procedures

Presentation transcript:

AGH University of Science and Technology Comparative analysis of aberration yields induced by radiation with different LET (Linear Energy Transfer) University of Warsaw Jakub Pietrzak UW University of Gdańsk Magdalena Rojek Monika Szymanska AGH University of Science and Technology Anna Kaminska Dominika Kedzierska Ewa Lagudza

THE DREAM TEAM

Laboratory of Radiation Biology Marta Deperas- Kaminska (supervisor) Ekaterina Zaytseva

The practice purposes: Theoretical introduction to cytogenetic methods used in biodosimetry Instructions of safety rules of work with human blood samples. To learn how to irradiate human lymphocytes and culture them under experimental conditions. Be able to carry out a simple experiment and analysis with irradiated cells

Standard light microscope SCHEME OF THE EXPERIMENT Incubation for the standard metaphase analysis colcemid 47h Fixation Staining with Giemsa 50h Standard light microscope Radiation

Our experiment Irradiation of blood samples with different LET radiation Lymphocytes culture (48 hours) Incubation for standard metaphase analysis Picture 2 Lymphocyte http://en.wikipedia.org/wiki/Image:SEM_Lymphocyte.jpg Picture 1 Blood http://en.wikipedia.org/wiki/Lymphocyte

Analysis of chromosomes aberrations Lymphocytes fixation Giemza staining Analysis of chromosomes aberrations Picture 3 Chromosomes http://humogef.ifj.edu.pl/zaklad/foto13.jpg Picture 4 Chromosomes 2 http://humogef.ifj.edu.pl/zaklad/foto13.jpg

Unstable aberrations - dicentrics, acentric fragments and centric and acentric rings in Giemsa stained metaphase Dic Centric ring Acentric ring deletion ace

RESULTS: The increase of aberration yields with increase of radiation LET in human peripheral blood lymphocytes is observed.

Acknowledgements Special thanks to: Dmitri Vladimirovich Fursaev Wladyslaw Chmielowski, Roman Zawodny Marta Deperas-Kaminska, Katia Zaytseva

THANKS FOR YOUR ATTENTION