Real-time Monitoring technology of Mass spectrometry Speaker : Achong

Outline IMS PTR-MS SIFT-MS Application of IMS IMS-TOF IMS-IMS-TOF Application of PTR-MS PIT-MS

Real-time Monitoring Mass spectrometry Environmental science Biochemical and medical diagnosis Workplace safety Security check

EI source Analysis of atmospheric air is especially challenging using electron ionization mass spectrometry, because the gas loading of the ion source by N2 and O2 (as well as water vapor) can be excessive. 70 ev

Real-time Monitoring MS Soft chemical ionization Measurement in Real-Time Without pre-concentration system

I. IMS (ion mobility spectrometer) Ion mobility mass spectrometry (Eiceman,1991; Baumbach, 2001) Analytical Chemistry, A390-A397, November 1, 2004

Ion mobility (K) Journal of Bimolecular Technique, Vol.13, Issue 2, June, 2002

Ion Mobility-Mass Spectroscopy Witt & Bowers: JACS. 2000, 122, 3458.

Ion mobility mass spectrometry Advantages: Multiform ion source could be applied. Some isomer could be separated. Real-time measurement Disadvantages: Lower mass resolution Lower sensitivity to allow accurate analysis of complex mixtures. Standard is requisite

II. PTR-MS (proton-transfer reaction) Proton-transfer reaction-mass spectrometry (Lagg A, Taucher J, Hansel A, Lindinger W. 1994.) http://info.uibk.ac.at/c/c7/c722/umwelt/html/animation.html

proton-transfer reaction H3O+ + R → RH+ + H20 [RH+] = [H3O+]o(1 - e-k[R]t) = [H30+]o[R]kt J. Mass Spectrom Ion Processes, 173 (1998) 191-241

J. Mass Spectrom Ion Processes, 173 (1998) 191-241

J. Mass Spectrom Ion Processes, 173 (1998) 191-241

PTR mass spectrometry Advantages: Soft ionization and low matrix effect Real-time measurement Low limit of detection (0.5 ppbv for 1-min measurements) Disadvantages: A large number of VOCs could not be identified and quantified.

III. SIFT-MS (selected ion flow tube) Adams NG, Smith D. 1976. Spanel P ,Smith D. 1995. http://www.syft.com/

SIFT mass spectrometry Advantages: application of multiple reagent ions (H3O+, NO+ and O2+) quantification in real-time discriminate isomers in Real-Time Disadvantages: The kinetic parameters of the reagent ion and the analyte ion must be known. Selected ion flow tube mass spectrometry for no-line trace gas analysis David Smith and Patrik Spanel Reference : Mass Spectrometry Reviews, 2005, 24, 661– 700

Application of IMS IMS/TOF IMS/IMS/TOF

Ion Mobility/TOFMS Anal. Chem. 2003, 75,6202-6208

Pulse sequence of IMS & TOF Anal. Chem. 2003, 75,6202-6208

example spectrum Analytical Chemistry, 1998 Vol. 70, No. 11, June 1,

Anal. Chem. 2003, 75,6202-6208

collision-induced dissociation http://proteomics.med.ncku.edu.tw/proteomicsweb/Liao_94_1007.ppt

IMS/IMS/TOF Anal. Chem. 2006, 78,4161-4174

operational mode A Anal. Chem. 2006, 78,4161-4174

operational mode B Anal. Chem. 2006, 78,4161-4174

operational mode C Anal. Chem. 2006, 78,4161-4174

Application of PTRMS PIT-MS

PIT-MS http://cires.colorado.edu/~gouw/Instruments/PITMS/PITMS.html

PIT-MS proton-transfer ion trap-mass spectrometry J Am Soc Mass Spectrom 2005, 16, 1316–1324

CID process on ion-trap MS http://www.chem.agilent.com/cag/Other/IonTrap_fig2.gif

calibration measurement of the PIT-MS J Am Soc Mass Spectrom 2005, 16, 1316–1324

single CID measurement of m/z 59 ambient air pure acetone pure propanal J Am Soc Mass Spectrom 2005, 16, 1316–1324

CID of propanal and acetone m/z=59 m/z=41 m/z=31 J Am Soc Mass Spectrom 2005, 16, 1316–1324