Alfred Day Hershey His best known experiment is Hershey Chase experiment Concerned the replication of genetic structure of viruses 4 Dec. 1908--- 22.

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Presentation transcript:

Alfred Day Hershey His best known experiment is Hershey Chase experiment Concerned the replication of genetic structure of viruses 4 Dec May 1997

Teaching and research at the Department of Bacteriology 1936 Held an instructorship in bacteriology and immunology Work together with Professor J. J. Bronfenbrenner Became assistant professor 1942 Became associate professor

What is T2 ? The T2 phage is a strongly toxic bacteriophage and tadpole-shaped virus small virus that infect only bacteria Contain 50% DNA and 50% protein

Contains linear double-stranded DNA Infect E-coli

tail fibers of the phage attach to the bacterial cell to initiate infection

Protein coat of the T2 carries the organ of attachment to bacteria.

Virus attach to the surface of their host cellVirus attach to the surface of their host cell (a) The infective cycle of T2 in E.coil

Inject the DNA into the cellInject the DNA into the cell (b)

DNA accumulate,other genes are transcribed and translatedDNA accumulate,other genes are transcribed and translated (C)

DNA and protein are assembled into complete virusDNA and protein are assembled into complete virus (d)

The cell breaks and releases its content of virusThe cell breaks and releases its content of virus (e)

Hershey Chase Experiment Radioactive Sulphur (35S) non-radioative DNA? Radioactive Phosphorous(35 P) radioactive DNA?

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