Online supplementary material. Profound and persistent decrease of circulating dendritic cells is associated with ICU-acquired infection in patients with.

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Online supplementary material. Profound and persistent decrease of circulating dendritic cells is associated with ICU-acquired infection in patients with septic shock. Intensive care medicine Grimaldi D 1,2,3,4 *, Louis S 2,3,4 *, Pène F 1,2,3,4, Sirgo G 2,3,4, Rousseau C 2,3,4, Claessens YE 2,3,4,5, Vimeux L 2,3,4 Cariou A 1,2, Mira JP 1,2,3,4, Hosmalin A 2,3,4, Chiche JD 1,2,3,4 § 1 Medical Intensive Care Unit, Hôpital Cochin, AP-HP, 27 rue du Faubourg Saint Jacques Paris, France. 2 Université Paris-Descartes, 22 Rue Méchain Paris, France. 3 Institut Cochin, INSERM U1016, 22 Rue Méchain Paris, France. 4 CNRS UMR 8104, 22 Rue Méchain Paris, France. 5 Emergency department, Hôpital Cochin, AP-HP, Paris, France § corresponding author: tel: , fax: ,

Supplemental figures captions Fig. S1: Dot plot of gating strategy. mDCs are CD45 +, lineage -, HLA-DR & CD11c double positive cells, pDCs are CD45 +, lineage -, HLA-DR & CD123 double positive. Corresponding isotype control for CD11c and CD123 (IgG1-APC and IgG1-PE respectively) were used. Typical example of healthy control (HC, upper panel) and patients with septic shock (SS, lower panel) are shown. Expression of HLA-DR is shown as fluorescence-activated cell sorter analysis and the corresponding MFI is indicated on the graph. Fig. S2: Evolution at day 1, 3 and 7 (D1, D3 D7) of HLA-DR MFI on circulating mDCs and pDCs in patients with septic shock (grey boxes) or non-septic shock (dashed boxes). Patients with less than 50 DCs/mL could not be analysed for HLA-DR expression. HLA-DR MFI decreased significantly in both groups and both DCs types (P=0.007 & P= for mDCs and pDCs of septic shock group, P=0.02 & P=0.01). Fig. S3: Evolution of day 1, 3 and 7 (D1, D3 D7) mDCs and pDCs counts in patients from the non-septic shock group with (dashed boxes), or without (dotted boxes) ICU-acquired infection. Fig. S4: Evolution of individual mDC (upper panels) and pDC (lower panels) counts in patients with septic shock, which have developed (left panels) or not (right panels) ICU-acquired infections (IAI). Fig. S5: Evolution of individual HLA-DR expression on mDCs (upper panels) and pDCs (lower panels) in patients with septic shock, which have developed (left panels) or not (right panels) ICU-acquired infections (IAI).

Fig. S1 HC IgG1-APC IgG1-PE mDC HLA DR MFI= lin HLA DR lin- CD123-APC pDC CD11c-PE mDC CD45 SSC PBMC IgG1-APC CD123-APC pDC CD11c-PE mDC SS IgG1-PE HLA DR MFI= mDC CD45 PBMC SSC lin lin- HLA DR

Fig. S2 MFI D1D7D3 pDC s N=35N=26N=30N=17 N=7 D1D7D mDCs N=43N=29N=36N=17N=21N=8 Septic shock group Non-septic shock group

Fig. S3 cells/ml pDCs D1D7D3 N=25N=4N=14N=3N=6N= D1D7D mDCs N=25N=4N=14N=3N=6N=2 Patients without IAI Patients with IAI

Fig. S4 mDC IAI+mDC IAI- pDC IAI+ pDC IAI- cells/ml D1D7D3 D1D7D3

Fig. S5 HLA-DR mDC IS+HLA-DR mDC IS- HLA-DR pDC IS+HLA-DR pDC IS- D1D7D3D1D7D3 MFI