Stable Isotope and Metabolomics Core Facility AECOM Diabetes Center The Einstein Stable Isotope & Metabolomics Core is within the NIH funded Diabetes Research Center at Einstein and anchors the CMCR Metabolomics Core. The Core uses stable isotope flux and metabolite profiling to help formulate and test hypotheses about the metabolic consequences of various changes in gene expression and protein function, in order to guide further integrative systems biology analyses of the underlying mechanisms in diabetes, insulin resistance, obesity, and diabetic complications. The Core objectives includes assessments of plasma, urine and tissue metabolite profiles for diagnosis/characterization of physiological and pathophysiological states. These include key metabolites in the glycolytic, gluconeogenic, pentose phosphate, and tricarboxylic cycle pathways, fatty acid oxidation and complex lipids, such as ceramides, diacylgylcerols, triacylglycerols (TAGs), and phospholipids. In the CMCR framework, the Core will use these methodologies to explore the specific effects that radiation has on the metabolic network of each organ, and to explore the metabolic consequences of protein modification, by radiation, on the function of the metabolic network.

CMCR-AECOM Stable Isotope & Metabolomics Core Location: Price 368 Director: Irwin Kurland, MD PhD Sandhya Narasimhan, PhD Lab Members: Albert Einstein College of Medicine OF YESHIVA UNIVERSITY Bhavapriya Vaitheesvaran, PhD Kirsten Hartil, PhD Hui-Min Zhang PhD Metabolite Profiling and Flux Analyses LC/MS Specialist Seahorse Extracellular Flux Analyses Mitochondrial Physiology Animal Research Coordinator

XF Applications in Cellular Bioenergetic Assays Mitochondrial Function In vitro Toxicity Cancer Fatty Acid Oxidation & Glycolysis Cell quality Neurodegenerative diseases Translation to Clinical Applications Cardiovascular research Obesity/diabetes XF96 Extracellular Flux Analyzer Flux Analyzer

LC/MS Analysis Simultaneous analysis of multiple metabolites Waters Aquity High resolution Chromatography Identification of unknown species by structure elucidation Multivariate Statistical Analyses LTQ-Orbitrap

Waters Xevo Tandem MS

Synapt G2 Linear Hybrid quadrapole Time of Flight MS with MALDI Synapt G2 itself Synapt G2 with MALDI front end

GC/MS Analysis of Metabolites Lipid profiling: Fatty acids DAGs, TAGs Cholesterol Metabolites from the glycolytic pathway, TCA cycle, pentose cycle and amino acids Stable isotope assessments of dynamic glucose disposal (SipGTT), lipogenesis ([ 2 H] 2 O or U- 13 C]lactate), glucose production ([U- 13 C]glucose), glycerol production/lipolysis ([2- 13 C]glycerol), Cori cycle ([U- 13 C]lactate)

Waters Premier GC-Time of Flight-MS