Enzymatic Browning: Activity of Fruit Homogenates

Slides:

Advertisements

Similar presentations

Estimation of Cholesterol Lieberman-Burchard Reaction

Advertisements

Estimation of Carbohydrate by Anthrone Method

Non-Enzymatic Browning: Millard Reaction

PH, Acids and Bases.

Slide 1 of 45  CRS Questions Follow:. Slide 2 of 45 The acid dissociation constant for hexanoic acid is 1.41x A buffer solution is prepared by.

Determination of the Isoelectric Points of Casein

Isolation of Casein from Milk

Calorimetric Determination of Glucose by the 3,5-dinitrosalicylic acid Method Several reagents have been employed which assay sugars by using their reducing.

Assignment: water.

The assay of Tissue Glycogen Glycogen is a polysaccharide of glucose which functions as the secondary short term energy storage in animal cells. It is.

Lesson 12: What Happens When Substances are Mixed with Water?

Apple Browning Project

Some factors affecting polyphenol oxidase activity

Lecture 162/28/05 4:30 TODAY Dr. William Shay TSC 006.

Isolating DNA from Fruit Sherry Adrianos Jacque Keele.

Dr. Samah Kotb Nasr Eldeen.  1. Total and conjugated bilirubin  2. ALT  3. AST  4. Total protein  5. GGT  6. ALP  7. 5′-nucleotidase  8. Albumin.

Analysis of fruit juices –I

The effect of inhibitor (Inorganic phosphate & Sodium fluoride) on the rate of an enzyme catalyzed reaction.

By Adam Wanetik, Spencer Olesky And also Daniel Broadie

Serial Dilutions. 0.2 g/mL NaCl Stock Solution NaCl STOCK solution Make a 0.2-g/mL NaCl STOCK solution - Add 2 g of sodium chloride to beaker and add.

Scanning spectrophotometry and spectrophotometric determination of concentration BCH 333 [practical]

Transition Metal Pigment Lab

Quantitative Determenation of Alanine aminotransferase (ALT) CLS 431.

Determination of Iron in Water

Unit 6 Enzymes. Biological catalysts –Often end in -ase Regulate the rate of chemical reactions in the cell –Lower the activation energy needed to start.

The Effects of HCl and NaOH on Catechol

Unit 10B Review Reg Chem When a solution sits out over a long period of time and water evaporates the concentration of the solution __________.

4.6 To prepare one enzyme immobilisation and examine its application

DETERMING CONCENTRATIONS OF SOLUTIONS. MOLAR Molar is mol solube/1 L solution Making molar solution 1)Add ½ of the total solvent 2)Add required amount.

Serial Dilutions Example!

Summary We are going to talk about catechol. We will also talk about the acids and bases and how it works on the catechol reaction. In addition, the discussion.

III. Molarity Topic 9 The Mole. A. Molarity Concentration of a solution. total combined volume substance being dissolved.

Section 4.5 Concentrations of Solutions. Concentration Amount of solute dissolved in a given quantity of solvent or solution Amount of solute = Concentration.

Student Directed Investigation – due dates. Due Dates February 13 – Methods & Materials Section due (in Google drive – shared with me) February 13 – Introduction.

Phase Two Titration Year 10 EEI by Mr H Graham Volumetric analysis is; A type of chemical analysis which depends on the accurate measurement of solution.

Question 1 What is the volume (in mL) of 18.0 M H 2 SO 4 is needed to contain 2.45g H 2 SO 4 ?

Solutions & Solubility Solution Preparation. Solution Preparation from a solid  Standard Solution = a solution for which the precise concentration is.

Enzyme Lab. Enzyme: Phenolase Reaction catalyzedReaction catalyzed Catechol + ½ O > 0-benzoquinone Catechol + ½ O > 0-benzoquinone.

Making Molar Solutions From Liquids (More accurately, from stock solutions)

DILUTION CALCULATIONS Molarity of Mixture = total moles of chemical in which we are interested total volume of mixture Dilute Solution – a solution with.

Physical Science Unit Classification of Matter.

Chemistry of Life Chapter 2. All Living Things Use Energy Energy in living things is converted from 1 form to another (chemical-physical-thermal etc.)

The effect of temperature on the rate of an enzyme catalyzed reaction. The effect of temperature on the rate of an enzyme catalyzed reaction. Amani Alghamdi.

Chemistry in Biology  The activation energy is the minimum amount of energy needed for reactants to form products in a chemical reaction.  Exothermic.

The effect of pH on the rate of an enzyme catalyzed reaction 322 BCH Exp (6)

Quantitative Estimation of Pentose

OBJECTIVES To measure ascorbic acid level in food products Identify the sources of ascorbic acid Master of extraction and determination of ascorbic.

Absorption spectrum and spectrophotometric determination of concentration Experiment 1.

Quantitative analysis of Carbohydrate

EXPERIMENT (5) Preparation and Properties of Buffer Solution

EXP.2 (Quantitative determination of Amylase activity) Introduction:- The purpose of this experiment is to study the enzyme amylase which is found in saliva.

Kinetics analysis of β-fructofuranosidase enzyme

The effect of temperature on the rate of an enzyme catalyzed reaction

Kinetics analysis of β-fructofuranosidase enzyme

KEY CONCEPT Life depends on chemical reactions.

Measuring the rate of the reaction

High Water Conc High Solute Conc Low Solute Conc Low Water Conc High Water Conc High Solute Conc Low Solute Conc Low Water Conc High Water Conc High.

Lactate dehydrogenase reaction

The common lysis solutions contain A. sodium chloride.

Kinetics analysis of β-fructofuranosidase enzyme

Lactate dehydrogenase reaction

AP Chemistry Podcast 1.4 Beer’s Law and Stoichiometry Problem Set

Making a standard solution

Preparing Solutions.

EDTA Procedure for using EDTA to determine Ca2+ and Mg2+ in Natural Waters Presented by Jeff Kovalesky.

Exp. Iron in Vitamin Tablet

Unit 3, Lesson 14: Dilutions

Measuring the rate of the reaction

Preparing Solutions by Dilution

Presentation transcript:

Enzymatic Browning: Activity of Fruit Homogenates

Enzymatic Browning: Activity of Fruit Homogenates

Enzymatic Browning: Activity of Fruit Homogenates * Control enzymatic browning by: Heat Elimination of O2 Control of PH Use chemical inhibitors.

1. Tissue extract preparation: 10g fruit + 100ml distilled water

1. Tissue extract preparation: ICE Buckner funnel

2. Measurement oxidation rate: * at PH = 5 and 25ºC Blank 0.5ml D.W. 1ml 0.5M catechol 10ml 0.01M citrate buffer pH 5 Set the wavelength to 420 nm

2. Measurement oxidation rate: * at PH = 5 and 25ºC Sample Test Mix Well 0.5ml enzyme solution 1ml 0.5M catechol 10ml 0.01M citrate buffer pH 5 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 Min A

3. Effect of pH changes on enzyme activity: * at PH = ? and 25ºC Sample Test Blank 0.5ml D.W. 0.5ml enzyme solution 1ml 0.5M catechol 10ml 0.01M citrate buffer pH ? 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 Min A

4. Effect of added Sodium Bisulphite and Sodium Chloride on enzyme activity: a. Effect of added sodium chloride on enzyme activity: Sample Test 0.5ml enzyme solution 0.2ml conc. NaCL 1ml 0.5M catechol 10ml 0.01M citrate buffer pH 5 Blank 0.5ml D.W. 0.2ml conc. NaCL 1ml 0.5M catechol 10ml 0.01M citrate buffer pH 5

4. Effect of added Sodium Bisulphite and Sodium Chloride on enzyme activity: b. Effect of added sodium bisulphate on enzyme activity: Sample Test 0.5ml enzyme solution 0.2ml conc. NaHSO4 1ml 0.5M catechol 10ml 0.01M citrate buffer pH 5 Blank 0.5ml D.W. 0.2ml conc. NaHSO4 1ml 0.5M catechol 10ml 0.01M citrate buffer pH 5

Results: pH = 4 pH = 3 pH = 6 pH = 5 pH = 7

Results: Activity (A2min-A1min) pH 3 4 5 6 7 Samar A. Damiati