Real-time multi-parameter spectroscopy and localization in three-dimensional single-particle tracking by Christian Hellriegel, and Enrico Gratton Interface Volume 6(Suppl 1):S3-S14 February 6, 2009 ©2009 by The Royal Society

Schematic of experimental setup. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

Modelled modulation versus distance lookup tables. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

(a–c) Retrieved positions from an immobile nanoparticle embedded in a sol-gel glass. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

An immobile nanometre-sized luminescent bead has been moved along a specified path in three dimensions. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

Latex beads (100 nm) in glycerine. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

Sequence of spectra from a mixture of labelled beads passing through the observation volume. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

(a,b) Image sequence showing a cross section of a cellular membrane. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

Trajectories of uPAR aggregates near the cell membrane. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society