Duong et al Figure 1 A InputsGST-Mdm2 HEGO HE11HE19HE15 HEGO HE11 HE19HE15 B HEGO A/BCEFD 1595 HE11 A/BEFD CEFD HE19 A/BC HE15 C D ER  I GST-Mdm2GST C.

Slides:

Advertisements

Similar presentations

Supplemental Figure 1 a % pulldown from input LNCaP/scrambled-siRNA Figure 1 A, ChIP analysis of AR and PHB binding to the PSA promoter in the LNCaP/scrambled-siRNA.

Advertisements

P-ERK Total ERK Mel888 CHL1 WM BRAFi (nmol/L) Supplemental Figure 2: Other surface molecules unaffected ns PD-L1 ns MCSP DMSO BRAFi Supplemental.

Supplementary Figure 1. DAXX S564A does not change Mdm2/p53 stability or p53 ‑ mediated gene expression. (A) U2OS cells stably transduced with pCDH-DAXX.

ER  HSP90 DMSO 200  M I3C30  M DIM200  M TRYPTOPHOL Supplemental Figure 1. DIM and Tryptophol fail to induce the downregulation of ER  seen with I3C.

Supplementary Figure 1. Suzuki et al. DAPI/BrdU BrdU 24 hrs 48 hrs 72 hrs.

Figure S UV- UV+ Relative expression levels of rhoB mRNA by qRT-PCR *

Nardella et al. Supplementary Fig. 1 Murine Rheb wt Line 47 Line 50 Log gene expression (a.u.) ******* 200 bp Founders

a c b S1. Lee et al., 2011 * RRT-binding PXDLS-binding NAD(H)-binding

Progesterone receptor

Supplementary Figure S1.

A B C MDA-MB-231 OHT: h FOXM1 PLK CDC25b ERα Tubulin

MCF-7: E2 E2(-4h)+OHT E2(-4h)+ICI (h)

IDH1 mutant glioma cells are sensitive to TMZ

Arterioscler Thromb Vasc Biol

10X dilution x ~10nM full – length competitor

Wild‐type p53 binds and represses Rad51 promoter.

A. D. B. C. - ΔNP63α - β-Actin IMEC - SUM MDA-MB IMEC

Nore1 enhances interaction between HIPK1 and Mdm2.

MEL23 and MEL24 inhibit Mdm2 and p53 ubiquitination in cells.

DEAR1 binds to SMAD3 and induces SMAD3 ubiquitination.

Knock-down of endogenous PINK1 abrogates Parkin Ser65 phosphorylation.

RYBP binds to MDM2. RYBP binds to MDM2. (A) COS7 cells (5 × 105 cells per well) were transfected with 10 μg of T7‐MDM2, Myc‐RYBP or both for 24 h. The.

Volume 22, Issue 12, Pages (December 2015)

Oliver I. Fregoso, Shipra Das, Martin Akerman, Adrian R. Krainer

Structural Basis for an Unexpected Mode of SERM-Mediated ER Antagonism

MUC1 Oncoprotein Stabilizes and Activates Estrogen Receptor α

Supplemental Figure 1 a b c d.

FIP200 maintains microRNA1198-5p expression via Ago2 in naïve T cells.

MED12 interacts with TDRD3 in a CARM1-dependent fashion.

Supplementary Figure 1 IP: IgG HA 3HA-E2F1: cIAP1: wt K

Supplemental Figure 1 WT ub ub K48R ub K63R hrs

MUC1 Oncoprotein Stabilizes and Activates Estrogen Receptor α

Volume 11, Issue 5, Pages (May 2003)

MED12 association with ncRNA-a is disrupted by loss of CARM1 and TDRD3

A Critical Role for Noncoding 5S rRNA in Regulating Mdmx Stability

Ribosomal Protein S7 Is Both a Regulator and a Substrate of MDM2

Volume 10, Issue 4, Pages (February 2015)

Volume 122, Issue 6, Pages (September 2005)

HOXB7 interacts with ERα and enhances expression of ER target genes.

A B Supplementary Figure S1 PC3 cells Vehicle 3β-Adiol

Differential p53 excitability between DSBs and UV results from differential regulation of Mdm2. Differential p53 excitability between DSBs and UV results.

HDAC11 regulates CCL2 expression by recruiting PU.1.

Figure S3. Zhang et al. A B Myc-SIRT1 - + sir2a+/+ sir2a-/-

HIPK2 decreases the stability of Notch1-IC through proteasome-dependent degradation. HIPK2 decreases the stability of Notch1-IC through proteasome-dependent.

CoCoA, a Nuclear Receptor Coactivator which Acts through an N-Terminal Activation Domain of p160 Coactivators Jeong Hoon Kim, Hongwei Li, Michael R Stallcup

FIP200 maintains microRNA1198-5p expression via Ago2 in naïve T cells.

USP2a regulates MYC expression through the MDM2–p53 axis.

Transcriptional Regulation of AKT Activation by E2F

NF-κB Is Required for UV-Induced JNK Activation via Induction of PKCδ

Sebastian Rumpf, Stefan Jentsch Molecular Cell

RIPA-based buffer system Harsh-conditioned system

Silencing Socs1 in the LLC line confers increased response to IFNγ in vitro. Silencing Socs1 in the LLC line confers increased response to IFNγ in vitro.

S. cerevisiae Bre1p confers resistance to apoptosis induced by H2O2.

FBXL19-AS1 acts as a sponge of miR-431-5p in lung cancer (A) The predicted binding sites of FBXL19-AS1 and miR-431-5p, and the mutant sites in mutant-type.

Oliver I. Fregoso, Shipra Das, Martin Akerman, Adrian R. Krainer

Generation of an inducible form of FOXN1.

Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional.

A B D C E F Supplementary Figure S4. Epping et al. IP: flag input IgG

Overexpression of GFP-fused PH domain of Gcs1p did not affect distribution of mitochondrial or ER markers or actin. Overexpression of GFP-fused PH domain.

Volume 22, Issue 12, Pages (December 2015)

VP16-E2 is a more potent transcriptional activator than Gal4-VP16.

Caffeine treatment restores MDM2- and proteasome-dependent degradation of mutant p53 in cancer cells. Caffeine treatment restores MDM2- and proteasome-dependent.

Selective delivery of pIC/PPHAffibody decreases the survival of HER2-overexpressing cells. Selective delivery of pIC/PPHAffibody decreases the survival.

by Naoe T. Nihira, Kohei Ogura, Kouhei Shimizu, Brian J

Pirh2 represses p73-dependent transactivation.

Platinum-based chemotherapy increased TXNIP expression, and overexpression of TXNIP enhanced the effectiveness of this therapy. Platinum-based chemotherapy.

The activation of PKC-δ and JNK1 is essential for doxorubicin-induced senescence. The activation of PKC-δ and JNK1 is essential for doxorubicin-induced.

Expression data from genes involved in regulation of transit through the cell cycle in response to treatment with E2 and OHT. A. Expression data from genes.

In the presence of human monocytes, estradiol increased breast cancer cell dissemination to the periphery via CCL2 and CCL5. In the presence of human monocytes,

Presentation transcript:

Duong et al Figure 1 A InputsGST-Mdm2 HEGO HE11HE19HE15 HEGO HE11 HE19HE15 B HEGO A/BCEFD 1595 HE11 A/BEFD CEFD HE19 A/BC HE15 C D ER  I GST-Mdm2GST C E2 OHT Luciferase activity Mdm2 ER  Input IP:Mdm Input IP:Mdm * Gal4 Gal-mdm2 + VP16 Gal-mdm2 + ER-VP16 Galmdm2 + VP16+ p53 Gal-mdm2 + ER-VP16+ p53 Gal-GRIP + VP16 Gal-GRIP + ER-VP16 I GST-LBD C E2 Mdm2 GST ER  p53 Mdm Input IP:ER  IP: IgG

Duong et al Figure 2 ER  C E2 A Actin MEFWTMEFdKO B C ER   C E2 Controlp53+Mdm2 Mdm D ER  Actin Mdm2 MG Actin ER  ER  Mdm2 w/o Estradiol CHX treatment (hrs) ER  + Mdm2 Actin Mdm2 overexpression - +

C A C E2 GST GST-AF2 Input Mdm2 Mdm2  RING Duong et al Figure 3 ER  (HE11) Actin Mdm ER  ER  + Mdm2 WT ER  ER  + Mdm2  RING Mdm2 WT Mdm2  RING Actin C E2 Mdm2 ER  Actin Mdm D Mdm2 - + ER  -Ub ER  IP: ER  IB:  Ub IB:  ER  ER  -Ub B

Duong et al Figure 4 C C E2 OHT ICI ControlUV C E2 OHT ICI ER  p53 Actin D A CMG UV ActD Apoptosis (AU) MG132 C E1 E2 E3 DES Control ER  Actin B C E1 E2 E3 DES p53 ER  Actin p53 C E2 ControlMG UVActD ER  p53 Actin

A E2 E2+UV Hours of chase ER  levels (% of control) Duong et al Figure 5 C  RITA Control 2h4h8h ER  p53 Actin B E2 +UV E2 CHX treatment (hrs) ER  p53 ER  p53

Relative luciferase activity CE2 C p53+Mdm2 MEFdKO B CUV Control E2 A CE2OHTICI MCF7BG1 CE2OHTICI D Duong et al Figure 6 Control Relative luciferase activity C E2 BG1 cells ER  Actin C E2 Control pS2 p53 actin C E2 UV C