Fig. S1. Z-stack of cells in a VZV vesicle smear. (A-H) Slices from bottom to top in increments of 1  m. Green = autophagosomes; red = VZV gE. LC3B gE.

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Fig. S1. Z-stack of cells in a VZV vesicle smear. (A-H) Slices from bottom to top in increments of 1  m. Green = autophagosomes; red = VZV gE. LC3B gE

Fig S2 A. Z-stack of MRC-5 fibroblast cells treated with tunicamycin for 24 h. Slices A1 – A5 from top to bottom in increments of 0.8  m. Note LC3B puncta indicative of autophagosomes A1A2 A3 A4 A5 LC3B DNA 50  m

Fig. S2 B. Z-stack of MRC-5 fibroblast cels treated with tunicamycin for 24 h. Slices B1 – B5 from top to bottom in increments of 0.5  m. Note LC3B puncta indicative of autophagosomes. B1B2 B3 B4 B5 LC3B DNA 50  m

Fig S2 C. Z-stack of MRC-5 fibroblast cells treated with tunicamycin for 24 h. Slices C1 – C5 from top to bottom in increments of 0.5  m. Note LC3B puncta indicative of autophagosomes. C1C2 C3 LC3B DNA C4 C5 50  m

D4D5 Fig S2 D. Z-stack of MRC-5 fibroblast cells Slices D1 – D5 from bottom to top in increments of 0.5  m. D1D2 D3 LC3B DNA 50  m

Fig S2 E. Z-stack of MRC-5 fibroblast cells Slices E1 – E5 from bottom to top in increments of 0.75  m. E4 E5 E1E2 E3 LC3B DNA 50  m

Fig S2 F. Z-stack of MRC-5 fibroblast cells Slices F1 – F5 from bottom to top in increments of 0.75  m. F4 F5 F1F2 F3 LC3B DNA 50  m

Fig S3. HeLa cells transiently transfected with PCMV_IE62 plasmid for 12 h. F E AB C LC3B IE62 DNA D 50  m

L K GH I J LC3B IE62 DNA Fig S3. HeLa cells transiently transfected with PCMV_IE62 plasmid for 12 h

MN O 50  m LC3B IE62 DNA Fig S3. HeLa cells transiently transfected with PCMV_IE62 plasmid for 12 h. Green = autophagosomes; red = VZV IE62 protein; blue = Nuclear dye.