Bio 414LS Week 2 Lecture: Normal Fly Development & Microscopy Activity: Disc dissection Lecture: Fly Genetics, Husbandry & Handling Activity: Virgining Assessment: Sabotage! Reading: Tyler Manual Chapter 8 Drosophila Guide Reading: Fly Pushing Chapter 1 Activity: Virgining Activity: Virgining Activity: Virgining The lecture and activity are to achieve these objectives: *Students will be able to briefly describe how a fertilized egg will develop into a 1 st instar larvae with regard to patterning, germ layers & germ cells. *Students will be able to describe the life cycle with regard to timing of stages. *Students will be able to identify the parts of an adult wing. *Students will be able to dissect and identify larval imaginal discs by shape and position. The lecture and activity are to achieve these objectives: *Students will be able to explain the relationship between genome sequence, genetic & cytological maps across the chromosome. *Students will be able to predict (in a very, very rough way) where their mutation lies on the genome by comparing the known genetic and/or cytological map position for their gene. *Students will be able to describe what an adult fly would look like given a genotype. *Students will be able to explain the differences between Drosophila and human dosage compensation *Students will be able to explain what balancers are used for and which is used for which chromosome. *Students will be able to sort and show the difference between sexes & virgins. *Students will be able to set up the light source & dissecting microscope.

Formative Assessment

Bio 414LS Week 2 Lecture: Normal Fly Development & Microscopy Activity: Disc dissection Lecture: Fly Genetics, Husbandry & Handling Activity: Virgining Assessment: Sabotage! Reading: Tyler Manual Chapter 8 Drosophila Guide Reading: Fly Pushing Chapter 1 Activity: Virgining Activity: Virgining Activity: Virgining The lecture and activity are to achieve these objectives: *Students will be able to briefly describe how a fertilized egg will develop into a 1 st instar larvae with regard to patterning, germ layers & germ cells. *Students will be able to describe the life cycle with regard to timing of stages. *Students will be able to identify the parts of an adult wing. *Students will be able to dissect and identify larval imaginal discs by shape and position. The lecture and activity are to achieve these objectives: *Students will be able to explain the relationship between genome sequence, genetic & cytological maps across the chromosome. *Students will be able to predict (in a very, very rough way) where their mutation lies on the genome by comparing the known genetic and/or cytological map position for their gene. *Students will be able to describe what an adult fly would look like given a genotype. *Students will be able to explain the differences between Drosophila and human dosage compensation *Students will be able to explain what balancers are used for and which is used for which chromosome. *Students will be able to sort and show the difference between sexes & virgins. *Students will be able to set up the light source & dissecting microscope.

Summative Assessment Big course objective to match: Students will be able to design an experiment to identify a mutation by mapping techniques and illustrate how that mutation in the genome could affect a protein and how that change could cause a phenotype. (Synthesis & Application)