Introduction The Oberlin College Living Machine (LM) is a wastewater treatment facility designed to model the functional processes of nutrient removal in wetlands The LM recycles all of the wastewater in the environmental studies building. The primary goals of the wastewater treatment are removal of nutrients (nitrogen and phosphorus), organic matter, and pathogens to prevent eutrophication in downstream bodies of water and harm to human health. Fecal coliform (FC) are a group of bacteria used as an indicator of pathogenic contamination in water and are measured to show whether microbial populations are being minimized by the LM. The LM is a more complex system than conventional wastewater treatment facilities because it utilizes microbial and plant communities that develop synergistically over time. We looked at FC and nutrient levels in the LM from the past three school years because the efficiency of the ecological processes in the LM is expected to reflect temporal, seasonal, and spatial patterns. Hypotheses 1.As microbial communities become more adept at processing nutrients over time, we expect to see decreasing concentrations of nutrients in the LM Effluent from 2004 to The Oberlin College schedule results in increased LM flow in spring and fall and decreased flow during summer and winter breaks. Thus, we expect a seasonal cycle of increased nutrients throughout the LM system during fall and spring semester. 3.Nutrients are removed by metabolic processes that vary in function and location in the LM. We expect NH 4 + to peak in the anaerobic tanks, and NO 2 - and NO 3 - to peak in the first aerobic tanks and decrease in the marsh due to ammonification, nitrification, and denitrification. PO 4 3- concentrations should remain constant throughout the LM until it is sequestered in the marsh by positively charged gravel. Cl - concentrations are expected to remain unchanging throughout the LM system because it is an inert ion. Temporal, Seasonal, and Spatial Patterns in Water Quality Indicators in a Wetland-based Wastewater Treatment System Systems Ecology (ENVS316) ‘06 Oberlin College, Ohio Molly Danielsson, Elyse Perruchon, Matthew Thayer Conclusions 1.Nutrient trends in the LM Effluent do not show the clear temporal patterns we expected. 2.Nutrient concentrations generally show seasonal patterns, increasing during school and decreasing between semesters. Patterns in NO 3 - concentrations are unexpected, as NO 3 - continues to increase in LM Effluent during winter break and spring semester We hypothesize that changes in the denitrification process responsible for metabolizing NO 3 - are causing increased Effluent concentrations of NO 3 -. Changes in denitrification could be caused by unfavorable conditions in the marsh such as low carbon supplies or the presence of oxygen. 3.Nitrogen trends agree with our spatial hypotheses. PO 4 3- follow the same pattern as NO 3 -, whereas we expected concentrations to remain constant until reaching the marsh. Cl - decreases throughout the LM unlike our hypothesis. As expected, FC levels decrease throughout the LM. Future Studies Further analysis of temporal patterns should include data from a longer time period. Further study should work to elucidate the relationship between phosphate and nitrate biochemical processes to maximize removal of both nutrients OA1 of the Oberlin College Living Machine Acknowledgments LM images thanks to Apostle Dyankov (OC ‘06) Sample Date /20/ /20/2004 3/20/2005 6/20/20059/20/ /20/2005 3/20/20066/20/2006 9/20/ /20/2006 Cl PO4 NO 3 - SO4 NO2 Nutrient concentrations in LM Effluent between 2004 and Discontinuous lines represent unsampled time periods. Results: nutrient concentrations decrease during school breaks and increase upon student return. NO 3 - continues to increase over winter break in 2006, while other nutrients remain stable throughout the year. FC and nutrient levels represent median values of data sets from school years 2004 through Results: NH 4 +, Cl -, SO 4 2- and FC concentrations were highest in AN1. NO 2 -, NO 3 - and PO4 peak in CA1. All variable decrease through subsequent tanks except for NO 3 - and PO 4 3-, which have smaller peaks in OA3. Ion Concentration Variability in LM Effluent over Time Ion Concentration (scaled to maximum value) Winter Break School Starts Living Machine Flow Diagram AN1 AN2 CA1 CA2 OA1 OA2 OA3 ToiletClarifier Post UV EffluentMarsh Methods We used Dionex Ion Chromatography to measure concentrations of NO 2 -, NO 3 -, PO 4 3-, SO 4 2-, and Cl - in samples taken weekly from the LM tanks between Fall 2004 and present. We measured NH 4 + concentrations with an Orion Ammonium Probe. Fecal coliform levels were determined with a FC detector test bi-weekly between 2004 and Most of our FC data was for AN1, CA1, Effluent, and Post UV. Key Ion and Fecal Coliform Levels by LM Tank AN1CA1OA1OA2OA3ClarifierMarshEffluentPost UV LM Tank Concentration (scaled to maximum value) NH 4 + (max value = 3.42 mol/L) Cl - (5.14E-02) NO 2 - (3.35E-04) PO 4 3- (5.54E-04) NO 3 - (2.10E-03) SO 4 2- (4.33E-03) FC (7.60E+08 CFU/mL)