Nutrient Analysis Using Colorimetry Torrey Lindbo SWRP, PSU-ESR.

Slides:



Advertisements
Similar presentations
Spectrophotometer.
Advertisements

S&S 12.1 Soil Nutrients and Fertilizers GLEs , 3.1.3,
Year of Clean Water: National Water Monitoring Day Water Testing Kits.
Can’t Judge a Powder by Its Color
Nutrient Analysis in the Wastewater Laboratory FVOA Mini-Conference 2012 Presenters: Mary Johnson & Connie Potter.
Determination of Concentration Using Spectrophotometry
Visuals, Spellings, and Uses The Right tool for the job!
Spectrophotometers and Concentration Assays
25.1 DISPOSING OF TOXIC HEAVY METALS PRE-LAB: PART A- “FIXING” THE COPPER IONS IN A SOLID MIXTURE.
SPECTROPHOTOMETRY IN BIOTECHNOLOGY
PH Study General Chemistry 101/102 Laboratory Manual University of North Carolina Wilmington.
TESTS ON AMINO ACIDS AND PROTEINS
Experiment 22: Colorimetric determination of an equilibrium constant
Heavy Liquids Separation. Heavy Liquids Overview Methylene iodide (MI; ρ = 3.32 g/cm 3 ) is used to concentrate material according to density. When added.
Chemicals and Apparatus: Putting the Tools to Work
8 reaction lab.
Radical Recombination Kinetics. Objectives To synthesize a dimer, which upon irradiation, undergoes dissociation to a radical Determine the order and.
Introduction to Spectrophotometry
Introduction to Spectrophotometry. Why Spectrophotometry? Imagine you are to make a 1μM solution of a specific protein that you believe could have anti-carcinogenic.
Introduction to Instrumental Analysis - Spectrophotometry
Lab 8: Soil Testing and Organic Matter
SPECTROPHOTOMETER Dr. Maha Al-Sedik. Principle:  The solutions of many compounds have characteristic colors.  The intensity of such a color is proportional.
Dr. Maha Al-Sedik. Objectives:  Electromagnetic Radiation  White light  Beer’ s law  Spectrophotometer  Components of spectrophotometer  Types.
COLORIMETRYCOLORIMETRY Prepared By Michigan Department of Environmental Quality Operator Training and Certification Unit Note: A printed description of.
Determination of Iron in Water
Colorimetry & Spectrophotometry.
SPECTROPHOTOMETRY. Determines concentration of a substance in solution by Measures light absorbed by solution at a specific wavelength by using spectrophotometer.
1 Spectroscopic ANALYSIS Part 5 – Spectroscopic Analysis using UV-Visible Absorption Chulalongkorn University, Bangkok, Thailand January 2012 Dr Ron Beckett.
Water Quality Testing. Temperature Why record temperature of water?: Different species of fish thrive in different water temperatures. A safe temperature.
Lab Equipment and Safety Procedures Yes, you should take notes!
Scientific Method Unit 2. We will set up the scientific method experiment today and will work on it for the next 2 weeks. Mealworm or Tenebrio molitor.
Visuals, Spellings, and Uses The Right tool for the job!
SPECTROPHOTOMETRY IN BIOTECHNOLOGY
Mr. Banerjee’s Science Classroom 1 Lab Safety Pages
Spectrophotometers and Concentration Assays
CONVERSION OF AN ALCOHOL TO AN ALKYL BROMIDE WITH REARRANGEMENT
Exp. 18: Spectrophotometric Analysis: Concentration of a Solution Using Beer’s Law White light violet (400nm) – red (800nm) “visible spectrum” sample (light.
What is a Watershed? An area of land, from ridge top to ridge top, that collects, stores, and releases water to a common point, such as a river or a lake.
Qualitative Analysis: Group I
Visuals, Spellings, and Uses The Right tool for the job!
SPECTROPHOTOMETRY. Spectrophotometry Determines concentration of a substance in solution –Measures light absorbed by solution at a specific wavelength.
CLS 332 CLINICAL INSTRUMENTAL ANALYSIS. A VISIBLE ABSORPTION SPECTROMETER.
Test tube A test tube, also known as a culture tube or sample tube, is a common piece of laboratory equipment consisting of a finger-length of glass tubing.
Determination of Concentration Using Spectrophotometry
Aqua Regia.
Photometry.
Laboratory Activity Two
SPECTROPHOTOMETRY IN BIOTECHNOLOGY. LIGHT IS A TYPE OF ELECTROMAGNETIC RADIATION Imagine electromagnetic radiation like waves on a pond – But instead.
Measuring Enzyme Activity Using Spectrophotometry (Beer’s Law)
Lab (9): Measurement of colors Spectrophotometry Analytical biochemistry lab KAU-Biochemistry dep. Nouf Alshareef
Spectrophotometry at a Glance
COLORIMETRY & SPECTROPHOTOMETR
Chem  Law Enforcement Used to prosecute drunk drivers with BAC’s above legal limit of 0.08%  Alcohol problems world wide problem  40 % of accidents.
Absorption spectrum and spectrophotometric determination of concentration Experiment 1.
Lab1 A VISIBLE ABSORPTION SPECTROMETER. -One of the simplest and most widely used methods to determine concentration of a substance in solution -Measures.
Spectrophotometry Measuring Concentrations of Substances in Body Fluids.
Introduction to Spectrophotometry
Lab1 A VISIBLE ABSORPTION SPECTROMETER
Safety Video SDS Information SDS Activity
Laboratory equipment Lecture (4).
Spectrophotometer Dr . S. Jayakumar.
Determination of the Amount of Copper in Brass
RINCIPLE OF COLORIMETER AND SPECTOPHOTOMETER AND VARIOUS TYPE OF ANALYSER USED IN CLINICAL BIOCHEMISTRY.
Lab 8: Soil Testing and Organic Matter
Colorimetry and Beer’s Law
Concentration determination of acetylsalicylic acid by using ultraviolet spectrometer Liang Zhang 03/25/2016.
Macromolecule Tests INB Pg 27.
Purpose of the Lab 3E, 3F, 3H Learn how to use a spec to figure out concentration of solutions Learn how to use a GLX Practice application of calculations.
Aqua Regia and Nitric acid Safety
Clinical instrumental analysis
Presentation transcript:

Nutrient Analysis Using Colorimetry Torrey Lindbo SWRP, PSU-ESR

Safety Equipment Gloves Goggles Material Safety Data Sheets Other Considerations: Alcohol Hand Wash Table or Tarp (spill prevention)

Colorimetry Colorimetry: method for quantifying the concentration of a chemical constituent by analyzing change in color Based on color change for standard solutions of known concentration Light usually filtered to specific wavelength Visible Light Spectrum = ROYGBIV Red ~ 700 nm Violet ~ 400 nm

Colorimeter Color change measured using colorimeter Colorimeter determines amount of transmitted light absorbed by sample Transmitted Light Detector Light source Tungsten Lamp Lens Sample Vial Filter

Light Paths Refracted Absorbed Transmitted Reflected Absorbance (A) is logarithmically related to transmittance (T) A = - log T

Beer’s Law A = εLc Where, A = absorbance ε = absorptivity; experimentally derived constant for each substrate L = path length of light through sample c = concentration of substance in solution

Beer’s Law Concentration (c) A = εLc Absorbance (A) Slope = εL

Hach DR/850 Colorimeter Has pre-programmed filters and calibration curves Cap doubles as light cover Sample color change compared to “blank” – what ZERO looks like Blank may be stream sample with no reagent added, or it may be deionized water with reagent I/ READ DR/850 Colorimeter TIMEREnter

Hach DR/700 Colorimeter Each test requires a “module” that needs to be turned to the correct program number. Example: Nitrate module = Low range procedure = program High range procedure = program Need to perform Lamp Intensity Adjustment Use film canister as light cover

“Zeroing” the Colorimeter Concentration (c) Beer’s Law A = εLc c = εL / A A Slope = εL (constant) Sample Absorbance Blank Absorbance

Other DR/850 Features Timer Beeps when reaction periods for tests are over Limits colorimeter use; no other tests can be run while timer is running Store Can store up to 99 readings Download Hach makes infrared computer interface

Hach Glassware Cleaning is crucial first step Acid Washing Soak in ~10% acid bath Choose acid depending upon tests (i.e., don’t use nitric acid if you’re testing for nitrate) Rinse thoroughly with DI water Etching on Hach glassware may erode after long soaks 10 mL 20 mL 25 mL

Colorimetric Analyses Soluble Reactive Phosphorus (SRP) Total Phosphorus Ammonia Nitrogen Nitrate Nitrogen Chlorine

Phosphorus Clean glassware crucial SRP test is quick and easy (2 minute reaction time) – good for flexibility with colorimeter sharing Total Phosphorus requires digestion Hot plate Strong Acid and Base Requires fume hood This test should be run in the lab

Phosphorus Equipment Needed 3-5 vials for samples + 1 for blank PhosVer3 reagent powder pillows Non-toxic waste container DR/850 Colorimeter Procedure Overview 10 mL sample + reagent in each sample 10 mL sample and no reagent for blank Shake and wait 2 minutes

The Meniscus Meniscus: the curved, lens-like surface of water in glass Glass has a negative charge that attracts the positive H from water Read volumes from bottom of meniscus 10 mL

Nitrate Nitrogen Vigorous Shaking 2 Procedures: Low (< 0.5 mg/L) and High (0.6 – 20 mg/L) Range Toxic waste – Cadmium (heavy metal) used to reduce nitrate Time requirement (i.e., Low Range takes 20+ mins.) Shake 3 min, wait 2, add reagent 2, shake, wait 15 min.

Low Range Nitrate Procedure Equipment Needed: 6-10 vials for samples + 1 for blank NitraVer6 and NitriVer3 reagent powder pillows Low Range Procedure: 30 mL sample + NitraVer6 in each sample (shake 3 mins) 10 mL treated sample + NitriVer3 10 mL sample and no reagent for blank

High Range Nitrate Procedure Equipment Needed: 3-5 vials for samples + 1 for blank NitraVer5 reagent powder pillows Toxic waste container High Range Procedure: 10 mL sample + NitraVer5 in each sample 10 mL sample and no reagent for blank

Ammonia Nitrogen Timing – measure each sample immediately after reaction time Time table; stagger reagent addition to each vial by 1 minute Blank is deionized water + reagents (only need 1 blank) DI water source must be PURE

Ammonia Nitrogen Equipment Needed: 3-5 vials for samples + 1 for blank Ammonia Salicylate and Ammonia Cyanurate reagent powder pillows Toxic waste container Procedure Overview: 10 mL sample + reagents in each sample 10 mL DI water + reagents for blank

Chlorine Sample needs to be collected in glass Best measured immediately in field

Chlorine Equipment Needed 3-5 vials for samples + 1 for blank DPD chlorine reagent powder pillows Non-toxic waste container DR/850 Colorimeter Procedure Overview 10 mL sample + reagent in each sample 10 mL sample and no reagent for blank Shake and wait 2 minutes

Waste Management Appendix A, pages 1-3 Collection Plastic Bottles work fine Clearly Labeled Non-Toxic, Ammonia and Nitrate all should be stored separately Organization “Callister Carrier” = cardboard 6-pack container

Non Toxic Waste DO, pH, Alkalinity, SRP, Chlorine Disposal Neutralize pH (baking soda) Pour down drain with excess water

Ammonia Waste Several constituents of concern Maximum concentrations in manual No way to safely reduce volume Must be disposed of at a hazardous waste facility METRO or PSU Waste Consortium Talk to your local waste handler, administrator or local college for other disposal sites

Nitrate Waste Cadmium is only constituent of concern Manual has instructions for precipitating heavy metals Most teachers bring entire waste container to hazardous waste facility