The CCA phage hunt 2013 Phage. Lab outline Isolate phage visualize phage Soil samples.

Slides:



Advertisements
Similar presentations
Aseptic technique. Before you start, get a bottle of O-SYL and wipe down your bench with it.
Advertisements

Aseptic Technique: Media and Equipment
Presentor: Jesus A. Hernandez Gregory-Portland High School Dr. Kung-Hui Chu Assistant Professor, Department of Civil Engineering.
Microbiological Methods
Biology Microbiology Kathleen Devlin
Tools of the Laboratory
Rhodococcus opacus What is the Research Question? Armando Vital Rivera High School Brownsville ISD Dr. Kung-Hui (Bella) Chu Assistant Professor, Department.
How To Prepare, Sterilize, AND Test Culture Media
Isolation technique with aseptic techniques to cultivate bacteria
Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms.
ASEPTIC TECHNIQUE Removing inoculum from a broth culture
Transformation of Escherichia coli
L8 – Keeping things sterile
ASEPTIC TECHNIQUE Removing inoculum from a broth culture
Experiment No 1. 5 Experiment Material and Chemicals Overview Introduction Procedure Objective
Recombinant DNA Bacterial Transformation Student Instructions Transformation.
Introduction to Lab Ex. 19: Enumeration of Bacteria
Culture Media Lab 2:.
Bacterial Transformation Teacher Preparation
How to inoculate culture media
Practical 7 ST anaerobic cultivation Bacteria in relation to oxygen Anaerobic infections - sampling and transport Cultivation - establishing of anaerobic.
Introduction to Lab 6: Ex. Preparation of Culture Media
Microbiological Methods
Starter: Aerobic respiration food (glucose) oxygen carbon dioxide water energy + + +
Growing Microbes L.O: To understand how microorganisms can be safely cultured in a laboratory Answers in your book: Antibiotics do not work on which type.
Aseptic Technique: Media and Equipment. Growth Medium A growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms.
Class 2 Review of Class One - Microscopy and Introduction to Aseptic Technique and Media Mrs. Sidelsky.
4-2 Sources of DNA.
Biosafety.  NO FOOD OR DRINKS!  Wash hands thoroughly  Disinfect counters and work area  Tie hair back  Smock, apron, or lab coat optional  Gloves.
Culturing Yeast Cells on Media. Pre Lab Definitions: Petri Dish: A round, shallow dish used to grow bacteria. Culture: To grow living organisms in a prepared.
Ex. 7: Aseptic Technique – Inoculating Different Types of Media Key concepts: 1.Different types of bacterial cultures: broth, agar slant, and agar deep.
Lab 2: Culture Media. In this lab we learn about different types of media that are used to grow bacteria. Some types of media will grow just about any.
Lab 18 Goals and Objectives: Exercise 21: Enumeration Work as two pairs per group (max 5 groups) Both pairs use same Escherichia coli culture Use pre-poured.
Isolation technique with aseptic techniques to cultivate bacteria
The Effect of Fertilizer Runoff on the Infectivity of T2 Bacteriophages Luke Diorio-Toth Grade 10 Pittsburgh Central Catholic HS.
General Microbiology Laboratory Sterile Technique & Pure Culture Concept.
Aseptic Technique Do you want a footer?.
Recombinant DNA Bacterial Transformation Student Instructions Mid-Log Culture Prep.
Gram Staining Microbiology How to Make a Smear  1. Label the frosted side of your slide with your initials, the name of the organism, and the date.
Ch6 Aseptic Technique “Contamination of microorganisms (bacteria, mycoplasma, yeast, fungus) remains a major problem in tissue culture” [see Table 19-1]
Isolation of bacteria by dilution plating
Chapter 8 – Bacterial and Viral Genetic Systems
Isolation of Bacteriophages
1. 2 Microbiology is the science that deals with the study of too small organism (micro-organisms) that are invisible to the naked eye.
Introduction In natural environments, microorganisms usually exist as mixed populations. However, if we are to study, characterize, and identify microorganisms,
Aseptic Technique This is how we grow bacteria and moulds without contaminating our cultures or ourselves. It is also called ‘sterile technique’. You must.
Bacterial identification plating streaking how to inoculate how to observe.
Science Projects using Bacteria Aseptic Lab Technique The following guidelines are meant to assist you in following proper sterile technique during bacteria.
Microbial Biotechnology Reem Alsharief Lab 3. General Methods of Isolation and selection of Microorganism Microbial isolation: To separate (a pure strain)
A Genomic Comparison of Cluster A Mycobacteriophages: Redefining Homoimmunity Amanda Scott D. Lovas, M. Richters, S. Bhuiyan, B. Miller, J Harmson C.R.
Microbiological Methods
DNA Transformation Lab E Coli with PCU. Avery–MacLeod–McCarty experiment Showed DNA is the substance that causes bacterial transformation.
Microbiological Tools To assist with transferring cultures to the different types of media there are a few different types of tools that can be utilized.
Basic Microbiology and Immunology Practical Course 2016.
Yellow Fever virus (Shutterstock/molekuul.be) Chikungunya virus (Shutterstock/molekuul.be) West Nile virus (Shutterstock/decade3d-anatomyonline) Dengue.
Transformation of Escherichia coli
Inoculation Incubation Isolation Inspection Identification 5/6/2018
Sterile Technique & Pure Culture Concept
How do we stop or slow down the problem of antimicrobial resistance?
PLASMID ISOLATION AND ANALYSIS Part I Overnight Culture Suspension
Exercise 21: Enumeration Work as two pairs per group (max 5 groups)
Lab
Bacteriophages.
Culture Media Lab 2:.
pure culture isolation
Transformation of Escherichia coli
Aseptic Technique & Streaking for Isolation
Transformation of Escherichia coli
Transformation of Escherichia coli
Transformation of Escherichia coli
Presentation transcript:

The CCA phage hunt 2013 Phage

Lab outline Isolate phage visualize phage Soil samples

The host: Mycobacterium smegmatis Soil bacterium Mycobacteria and their phage are found in rich organic soil and mulch piles. Aerobic Hardy, resistant to: toxic compounds desication Harmless relative of the bacterium that causes tuberculosis

Bacteriophage = bacteria eater

Interesting facts about phage phage on earth infections/second Largest unexplored reservoir of genetic diversity Transfer genes between bacteria (transduction)

A gallery of mycobacteriophage

Genomics reveals that these 60 phage group into 9 major clusters, plus 5 singletons (phage that do not have sequenced relatives):

Sterile/Aseptic Technique Sterile: An environment (solution, tool) that is free of living organisms and viruses Aseptic: A process that is performed under sterile conditions

1. To grow only the desired phage and bacteria, rather anything that can find its way to your petri dishes! The goals of sterile technique: 2. To prevent cross contamination of your phage samples 3. To minimize exposure to the phage and bacteria we work with

Things to remember Always know where your hands are. Never pass your hands or fingers over the top of a sterile field (open bottles, flasks, tubes and bottle caps, and agar plates). Never have more than one tube, bottle, or flask open on the bench at a time. Never set a bottle or tube cap or Petri dish lid on a bench top. Never turn the caps or lids UP.

More things to remember Always work with an open flame when opening sterile tubes or bottles. Never go into a sterile solution with a used pipette or tip. Change tips every time! The shaft of a pipetman is not sterile. Do not introduce it into a tube or bottle!

Start the phage hunt

Enrichment A method that favors the growth of certain organisms from a complex mixture, such as soil or water. The Gulf oil spill Enriched for oil- degrading bacteria that used the oil (as carbon & energy source)

Mycobacterium culture Our enrichment protocol Environmental sample Environmental sample Clear solution Filter Provides mycobacteria for mycobacteriophage to infect, so they will outcompete other phage

Enrichment protocol (part 1) Transfer ~1 gram of each sample to your flask Pour culture solution and broth into flask Label the flask with your initials, date, period, and sample name Cover top with aluminum foil and place on shaker overnight