HEXOKINASE Korry Novitriani 20510045. Pendahuluan Heksokinase EC. 2.7.1.1 (2) menunjukkan nama kelas (transferase), (7 ) subkelas (fosfotransferase) dan.

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Presentation transcript:

HEXOKINASE Korry Novitriani

Pendahuluan Heksokinase EC (2) menunjukkan nama kelas (transferase), (7 ) subkelas (fosfotransferase) dan (1) sub-sub kelas (fosfotransferase dengan gugus hidroksil sebagai penerima), (1) D-glukosa sebagai penerima gugus fosfat.

Hexokinase Reaksi hexokinase telah ditemukan di setiap organisme Glukosa ATP

Reaksi Heksokinase

Karakteristik Hexokinase Ukuran : 50 kD kD pH Optimum : 7,5 - 9,0 Titik isoelektrik: PI, 5,25 dan P-II, 4,93 Stabilitas: suspensi kristal yang stabil selama 6-12 bulan 2-8 ° C. Aktivator : Mg 2+ Inhibitor : Glukosa-6-Phospat

Jenis Heksokinase Heksokinase I Heksokinase II Heksokinase III Heksokinase IV

Struktur Heksokinase

Preparasi Sampel

Isolasi Heksokinase Sampel Triton X-100 Percoll/ NaCl NanoLC/ESI/MS/MS

Kemurnian

Daftar Pustaka Toshihiko Aki and Shuichi Yanagisawa. “Application of Rice Nuclear Proteome Analysis to the Identification of Evolutionarily Conserved and Glucose-Responsive Nuclear Proteins”. Department of Applied Biological Chemistry, The University of Tokyo, Japan, and Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi, Saitama,Japan Mioara Larion and Brian G. Miller. “Global Fit Analysis of Glucose Binding Curves Reveals a Minimal Model for Kinetic Cooperativity in Human Glucokinase”. Department of Chemistry and Biochemistry, The Florida State University, Tallahassee, Florida. 2010