Figure S1 a. Neutrophilsb. Non-inflammatory monocytes c. Macrophages or DCs d. Inflammatory monocytes SSC FSC B C SSC * * * * * III Count F4/80 A.

Slides:

Advertisements

Similar presentations

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.

Advertisements

Counts TLR3-PE Counts TLR8-PE Counts TLR9-PE C AB Supplemental Figure 1.

Poster will be available at after September 10 th 2006 AN0128 Inhibits Pro-inflammatory Cytokine Production in a Macrophage Cell Line by.

Figure S1 A a b c 10× Ikkβ WT Ikkβ Mye  /  B C n=5, p

Pathogen invasion MM Neutrophil DC PRR Naïve T cell CTL IFN-  B cell IgG IL-4, IL-5, IL-6 Th1 Th2 IgE IgA Cell lysis Tr cell IL-1 , TNF- , chemokines.

SUPPLEMENTAL MATERIAL AND METHOD Ag-presenting CpG-activated pDCs prime Th17 cells that induce tumor regression Leslie Guéry, Juan Dubrot, Carla Lippens,

FSCCD40 mediumFluCL %82 %97 % SSC FSC GEN2.2 cells B mediumFluCL097CpG ACpG B GEN2.2 GENshTLR7 % CD40 positive cells TLR7TLR9 0.0.

1 Supplementary figure 1 CD64MHCIIF4/80CD24CD11c FMO Macrophages CD11b + DCs CD103 + DCs Steady state b c Hh +anti-IL10R uninfected ** n.s.

1 Cluster of differentiation molecules.. 2 CELLSCD Number Macrophages, monocyte CD4, CD11b, CD64, CD115, CD86 (B7-2), CD80 (B7 – 1) Dendritic cell CD1a,

Supplementary Figures NormalCRC CRC+5FU CRC+CPT11 100µm S1.

Anna Rydén, Maria Faresjö Diabetes Research and Clinical Practice

M1 – Immunology CYTOKINES AND CHEMOKINES March 26, 2009 Ronald B

M1 – Immunology EFFECTOR T CELL FUNCTIONS (Part I) March 27, 2009 Ronald B. Smeltz, Ph.D. Microbiology and Immunology

From: Neutralization of Ocular Surface TNF-α Reduces Ocular Surface and Lacrimal Gland Inflammation Induced by In Vivo Dry Eye Invest. Ophthalmol. Vis.

Figure 6. The effect of GV1001 on ENO1-induced pro-inflammatory cytokine production in RA PBMCs. RA PBMCs were pre-treated with GV1001 (100 µM) for 1 h.

Alexander Loewer, Galit Lahav Cell

Invest. Ophthalmol. Vis. Sci ;49(4): doi: /iovs Figure Legend:

Volume 85, Issue 4, Pages (April 2014)

The Th17 immune response in renal inflammation

Production of chemokines and proinflammatory and antiinflammatory cytokines by human alveolar macrophages activated by IgE receptors Philippe Gosset,

Cytokine and chemokine expression of flucloxacillin-specific T cell clones (TCC) of patient P1. Cytokine and chemokine expression of flucloxacillin-specific.

Volume 137, Issue 6, Pages e2 (December 2009)

Peter J. Barnes, FMedSci, FRS

IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft- Versus-Host Disease Doanh Le Huu, Takashi Matsushita, Guihua Jin, Yasuhito.

Volume 37, Issue 6, Pages (December 2012)

بلوغ و تمایز لنفوسیت های B

Yiping Wang, Yuet-Ching Tay, David C.H. Harris Kidney International

Figure 3 Cascade of events leading from AKI to ALI

B-Cell-Directed Therapy for Inflammatory Skin Diseases

Volume 36, Issue 1, Pages (January 2012)

Volume 87, Issue 2, Pages (February 2015)

Functional Beta2-Integrins Restrict Skin Inflammation In Vivo

Protective Effect of MFG-E8 after Cutaneous Ischemia–Reperfusion Injury Akihiko Uchiyama, Kazuya Yamada, Buddhini Perera, Sachiko Ogino, Yoko Yokoyama,

Supplementary Figure 1 FSC-A SSC-A HC UC CD

Figure 3 Biologics that attenuate effector responses in the kidney

Volume 140, Issue 2, Pages e4 (February 2011)

Human MSC Suppression Correlates With Cytokine Induction of Indoleamine 2,3- Dioxygenase and Bystander M2 Macrophage Differentiation Moïra François, Raphaëlle.

John T. Chang, William J. Sandborn Gastroenterology

Jethe O. F. Nunes, PhD, Juliana de Souza Apostolico, MSc, David A. G

Systemic Anti-TNFα Treatment Restores Diabetes-Impaired Skin Repair in ob/ob Mice by Inactivation of Macrophages Itamar Goren, Elke Müller, Dana Schiefelbein,

Volume 57, Issue 2, Pages (October 2000)

Supplementary Figure S1

Profiles of anti-inflammatory cytokines after endotoxin injection.

Epithelial Overexpression of SOCS-3 in Transgenic Mice Exacerbates Wound Inflammation in the Presence of Elevated TGF-β1 Andreas Linke, Itamar Goren,

Supplementary Figures

Volume 119, Issue 3, Pages (September 2000)

Viperin−/− mice have high levels of pro-inflammatory cytokines but low levels of Th2 cytokines. Viperin−/− mice have high levels of pro-inflammatory cytokines.

Exacerbation and Prolongation of Psoriasiform Inflammation in Diabetic Obese Mice: A Synergistic Role of CXCL5 and Endoplasmic Reticulum Stress Noriko.

IL-17A enhances IL-13 activity by enhancing IL-13–induced signal transducer and activator of transcription 6 activation Sara L. Hall, MS, Theresa Baker,

Volume 18, Issue 11, Pages (March 2017)

Romain Gallet et al. BTS 2016;1:14-28

CD4+ T lymphocytes mediate acute pulmonary ischemia–reperfusion injury

Fig. 4 Surgery initiates a systemic inflammatory response that triggers the outgrowth of distant immunogenic tumors and can be inhibited by perioperative.

Volume 34, Issue 5, Pages (May 2011)

Regulatory B Cells: Origin, Phenotype, and Function

Javed Mohammed, Andrew Ryscavage, Rolando Perez-Lorenzo, Andrew J

Molecular profiles of lung, and of blood and plasma in M

IL-10 and Macrophages Orchestrate Gut Homeostasis

Toll-Like Receptor Activation during Cutaneous Allergen Sensitization Blocks Development of Asthma through IFN-Gamma-Dependent Mechanisms Rita Haapakoski,

Anti-inflammatory cytokine production is increased in trained monocytes. Anti-inflammatory cytokine production is increased in trained monocytes. (A) IL-10.

Volume 20, Issue 7, Pages (July 2012)

Volume 137, Issue 6, Pages e2 (December 2009)

Volume 86, Issue 5, Pages (November 2014)

A Cell type Wild type Nox4-/- CD4+ T cells CD8+ T cells CD4- CD8- NK

A Metabolic Roadblock in Inflammatory Macrophages

Figure 3 Within-group comparisons (before–after)‏

Inflammation Mediated by JNK in Myeloid Cells Promotes the Development of Hepatitis and Hepatocellular Carcinoma Myoung Sook Han, Tamera Barrett, Michael.

Combination of R848 and anti-CD200R affects activation of tumor-infiltrating myeloid cells. Combination of R848 and anti-CD200R affects activation of tumor-infiltrating.

Volume 27, Issue 5, Pages (May 2019)

Presentation transcript:

Figure S1 a. Neutrophilsb. Non-inflammatory monocytes c. Macrophages or DCs d. Inflammatory monocytes SSC FSC B C SSC * * * * * III Count F4/80 A

A B Densitometry unit pNF-ĸB p65 pSTAT3 Figure S2 IKKβ ΔMye C57BL/6 Densitometry unit pSTAT3 pNF-ĸB p65

PBS (3 hours) EdTx (6 hours) C57BL/6 IKKβ ΔMye Treatment (time) EdTx (3 hours) C57BL/6 IKKβ ΔMye C57BL/6 IKKβ ΔMye Figure S3

CCL2 CXCL2 * * * * * * * * * * Relative mRNA levels (20 ― ∆Ct) Figure S4 Relative mRNA levels (20 ― ∆Ct) B A

* Ab titers (Log 2 GMTs) PA-specific neutralizing Abs Ab titers (Log 2 GMTs) PA-specific serum IgG titers A * B Figure S5

F1V-specific Ab titers (Log 2 GMTs) C57BL/6 IKKβ ΔMye Figure S6 Serum IgA F1V-specific Ab titers (Log 2 GMTs) Fecal IgA A B C57BL/6 IKKβ ΔMye

Figure S7 CD11b + F4/80 – Gr-1 high (Neutrophils) Frequency (%) in myeloid cells Days after injection A B CD11b + F4/80 – Gr-1 high (Neutrophils) Frequency (%) in myeloid cells CD11b + F4/80 + Gr-1 high (Inflammatory monocytes) CD11b + F4/80 + Gr-1 low (Macrophages or DCs) Frequency (%) in myeloid cells CD11b + F4/80 + Gr-1 ─ (Non-inflammatory monocytes)

Figure S8 Frequency (%) B220 + IL-17RA low B220 + IL-17RA high B ▼ * * ▼ ▼ * ▼ * C57BL/6 CD11b + IKKβ ΔMye CD11b + EdTx + – – – + – – – + + – – – + – – – + FSC IL-17RA A IL-17RA low IL-17RA high APRIL BAFF AID * ▼ ▼ ▼ ▼ * ▼ ▼ Relative mRNA levels (20 ― ∆Ct) C57BL/6 CD11b + IKKβ ΔMye CD11b + EdTx + – – – + – – – + C

Pro-inflammatory cytokine mRNAs (20 ― ∆Ct) Anti-inflammatory Cytokine mRNAs (20 ― ∆Ct) IL-1βIL-6 C57BL/6 CD11b + IKK  Δ Mye CD11b + ++–– +–+– ––++ EdTx ++–– +–+– ––++ * ▼ ▼ ▼ ▼ Enzyme mRNAs (20 ― ∆Ct) Caspase-1 * ▼ IL-10 TGF-  * * ▼ ▼ Figure S9 TNF-αIL-23 ▼ ▼ * * ▼ * C57BL/6 CD11b + IKK  Δ Mye CD11b + ++–– +–+– ––++ EdTx ++–– +–+– ––++ C57BL/6 CD11b + IKK  Δ Mye CD11b + ++–– +–+– ––++ EdTx A B C