GlnR. Schreier et al. 1991 J. Mol. Biol. 220:241 Gutowski and Schreier 1992 J. Bacteriol 174:671 Brown and Sonenshein 1996 J. Bacteriol 178:2450 Zalieckas.

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Presentation transcript:

GlnR

Schreier et al J. Mol. Biol. 220:241 Gutowski and Schreier 1992 J. Bacteriol 174:671 Brown and Sonenshein 1996 J. Bacteriol 178:2450 Zalieckas et al J. Bacteriol 188:2578 GlnR controls transcription of glnRA operon in B. subtilis via two binding sites. TnrA does not act upon the first site.

lactococcus/ streptococcus lactobacillus/ pediococcus/ enterococcus macrococcus/ staphylococcus Alignment of the upstream region of the glnRA operon in various species of the class Bacilli bacillus geo- paeni- lysini- oceano- anoxy- leuconostoc/ oenococcus listeria GlnR

Bacilli (18) Listeria (3) Staphylococci (7) Lactobacilli (8) Leuconostoc (3) Streptococci (12) GlnR binding motifs derived from the alignment of the upstream region of the glnRA operon in various species of the class Bacilli Eight sequence motif

>B1 ATGTAAGAAAAACTAACAT >B2 ATGTAAGAAAAACTAACAT >B3 ATGTAAGAAAAGCTAACAT >B4 ATGTTAGAAAAGCTAACAT >B5 ATGTTAGGAATCCTTACAT >B6 ATGTTAAGATTCCTTACAT >B7 ATGTGAAGATTTGTGACAT >B8 TTGTGATTTTTTGTGACAT >LIS1 ATGTTAGATTATCTTACAT >LIS2 ATGTTAGATTATCTTACAT >LIS3 ATGTTAGATTATCTTACAT >LIS4 ATGTTAGATTATCTTACAT >LIS5 ATGTTAGATTATCTTACAT >LIS6 ATGTTAGAAAATCTTACAT >LIS7 ATGTTAGAAAATCTTACAT >LIS8 ATGTTAGAAAATCTTACAT >Staph1 ATGTAAGATTTCCTTACAT >Staph2 ATGTAAGATTTCCTTACAT >Staph3 ATGTTAGAAAACCTTACAT >Staph4 ATGTTAGAAAACCTTACAT >Staph5 ATGTTAGAAAACCTAACAT >Staph6 ATGTTAGAAAACCTAACAT >Staph7 ATGTCAGGAAACCTGACAT >Staph8 ATGTCAGGAAACCTGACAT >LAB1 ATGTTAGAAAATCTAACAT >LAB2 ATGTCAGAAAACCTAACAT >LAB3 ATGTCAGAAAAGCTTACAT >LAB4 GTGTGATATTTTCTAACAT >LAB5 ATGTAAGAAAACCTTACAT >LAB6 ATGTCAGAAAACCTAACAT >LAB7 GTGTAAGAAAATATTACAT >LAB8 TTGTGAGTTTTTCTAACAT >LEU1 ATGTTAGAAAACCTAACAC >LEU2 ATGTTAGAAAACCTGACAC >LEU3 ATGTTAGAAAACCTGACAC >LEU4 ATGTTAGAAAACCTAACAC >LEU5 ATGTTAGAAAACCTAACAC >LEU6 ATGTTAGCTTTCCTAACAT >LEU7 ATGTTAGCTTTCCTAACAT >LEU8 ATGTTAGCTTTCCTAACAT >STR1 ATGTCAGAAAATCTAACAT >STR2 ATGTCATAAAATCTAACAT >STR3 ATGTCAGAAAATATAACAT >STR4 ATGTAAGAAAATATAACAT >STR5 ATGTAAGAAAATATCACAT >STR6 ATGTTATATAATATCACAT >STR7 ATGTTACATTTCGTGACAT >STR8 GTGTTACTTTTAGTGACAT Summary page of eight sequence motif bacilli (- streptococci)streptococci >1 ATGTTAGAAAATCTAACAT >2 ATGTTAGAAAATCTAACAT >3 ATGTCAGAAAATCTTACAT >4 ATGTCAGAAAACCTTACAT >5 ATGTAAGAAAACCTCACAT >6 ATGTAAGATTACATCACAT >7 ATGTGATATTTGATGACAT >8 GTGTGACGTTTAGTGACAT Listeria and Leuconostoc left out because of small sample size

TnrA

>amtB ATGTCAGGAAATCTTACAT >ansZ/yccC CTGAGAGAAAAGCTGACTG >gabP CTGGTATATTTTCTTACAC >glnQ TGGGTATATTTTCTGACAA >nasA GTGTCACAAAAACTTACAC >nasB GTGTAAGTTTTTGTGACAC >nasD TTGTTACATTTTATAACAT >oppA ATGGAAGAAAAACTAACGA >pucJ TTGTTACATTTTCTTACAA >pucR ATGTCAGTTTATGTAACAC >ykzB ACGGAAGAAAAATTAACAA >ywrD TCGTCAGTTTTTCTGCCGG Activating sites TnrA in B. subtilis

amtB

pucR