Lab session 2. Use and care of the microscope Ocular lens (10X) Objective lens 4X, 10X, 40X and 100X ( oil immersion objective) Total magnification =

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Presentation transcript:

Lab session 2

Use and care of the microscope Ocular lens (10X) Objective lens 4X, 10X, 40X and 100X ( oil immersion objective) Total magnification = magnification by the objective lens X magnification by the ocular lens (4) (10) = 40

As the magnification increases microscopic field decreases 10X to locate object – see most of the slide Center the object in the field 40X Fine focusing to sharpen the image Drop of oil immersion on the slide

Bring 100X place Fine focusing to sharpen image Make diagrams and write descriptions

Bacteria exists in 3 basic shapes Cocci/coccus – spherical Bacilli/bacillus – rod shaped Spirillum – loose spiral Spirochete – tight spiral

Bacteria exists in 3 major shapes chains Groups of two clusters

Groups of two chain

Budding yeast

Borrelia burgdorferi