8 seqs/day 96 seqs/2 hrs Bioinformatics for Genomics
TAGAGCATCGATCGATGCTGCAGATGATGCTAGCATCGGCTAGGCGACG ATCTCGTAGCTA ATCTCGTAGCTAGCTACGACGTCTA ATCTCGTAGCTAGCTA ATCTCGTAGCTAG ATCTCGTAGCTAGC ATCTCGTAGCTAGCT ATCTCGTAGCTAGCTAC ATCTCGTAGCTAGCTACG ATCTCGTAGCTAGCTACGA ATCTCGTAGCTAGCTACGAC ATCTCGTAGCTAGCTACGACG ATCTCGTAGCTAGCTACGACGT ATCTCGTAGCTAGCTACGACGTC ATCTCGTAGCTAGCTACGACGTCT ATCTCGTAGCT A G C T A C G A C G T C T A
Random base calling at the beggining or the end of read (Phred < 10) Trimming (trim or trim_alt algorithms) Phred does the base calling chromatogram acgatctcgctagctgctactgtagccgcgattattcgcgatctacgtatatcgcgatcgatc Each base has assigned a chance of failure 1% = 0,01 = = Phred 20 Base calling & trimming
Start End
Goal: To document the presence of transcripts in a transcriptome [otorrin... in portuguese] EST = Expressed Sequence Tag Partial sequencing of transcripts in EST genome projects actgatcatctcgctgatgcgatc work