8 seqs/day 96 seqs/2 hrs Bioinformatics for Genomics.

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8 seqs/day 96 seqs/2 hrs Bioinformatics for Genomics

TAGAGCATCGATCGATGCTGCAGATGATGCTAGCATCGGCTAGGCGACG ATCTCGTAGCTA ATCTCGTAGCTAGCTACGACGTCTA ATCTCGTAGCTAGCTA ATCTCGTAGCTAG ATCTCGTAGCTAGC ATCTCGTAGCTAGCT ATCTCGTAGCTAGCTAC ATCTCGTAGCTAGCTACG ATCTCGTAGCTAGCTACGA ATCTCGTAGCTAGCTACGAC ATCTCGTAGCTAGCTACGACG ATCTCGTAGCTAGCTACGACGT ATCTCGTAGCTAGCTACGACGTC ATCTCGTAGCTAGCTACGACGTCT ATCTCGTAGCT A G C T A C G A C G T C T A

Random base calling at the beggining or the end of read (Phred < 10) Trimming (trim or trim_alt algorithms) Phred does the base calling chromatogram acgatctcgctagctgctactgtagccgcgattattcgcgatctacgtatatcgcgatcgatc Each base has assigned a chance of failure 1% = 0,01 = = Phred 20 Base calling & trimming

Start End

Goal: To document the presence of transcripts in a transcriptome [otorrin... in portuguese] EST = Expressed Sequence Tag Partial sequencing of transcripts in EST genome projects actgatcatctcgctgatgcgatc work