Astrocyte Analysis By Masters in Computer Science Rakesh Singrikonda [rsingrik@kent.edu] Rambabu Chelikani [rchelika@kent.edu] Manoj Thatikonda [mthatiko@kent.edu] Masters in Computer Science Kent State University

Table of contents Astrocyte Structure Goals Segmentation and approaches. Our process.

What is astrocyte? These are star-shaped glial cells in the brain and spinal cord. These are the most abundant cell of the human brain. Astrocytes are a sub-type of glial cells in the central nervous system.

structure

Goals: automatically segment Individual Glial Cells. intelligent thresholding. Segmentation. seed points classification. classify astrocytes and provide volume and surface area.

Segmentation it is the process of partitioning a digital image into multiple segments. it is used to locate objects or their boundaries.

Approaches to segmentation: Simple tresholding. Edge detection. Watershed transformation. Etc….

Simple tresholding How To Choose The Value For The Threshold T ? By Visual Inspections Based On The Accurate Image Level The Treshold Can Be Applied. Does not require specific knowledge about the image.

Edge detection Edge Detection Extracts The Boundaries Of The Objects, Instead Of The Objects Themselves. Edge detection aims at identifying points in a digital image at which the image brightness changes sharply or more formally has discontinuities. Discontinuities in image brightness are likely to correspond to: discontinuities in depth discontinuities in surface orientation changes in material properties variations in scene illumination

Watershed transformation Watershed Segmentation Is An Approach Developed To Solve The Very Common Problem Of Separating Touching Objects. Segmentation Failed To Separate Too Close Objects

OUR Process Segmentation Thresholding, edge detection, watershed transformation

Cells after segmentation

Center of mass map

Centroid identification

Test Results

3D identification results

3D identification results

Summary of results

Any Queries ???