Corbett Research CAS-1200 Dimensions Operates from PC or laptop 490 (W) x 580 (D) x 390 (H) Operates from PC or laptop Manufactured and supported in Brisbane

Pipetting Single pipetting head Pipette volumes as low as 1 µL Repeatable to within 0.1 µL 50 µL and 200 µL tips Filtered Conductive tips

Level Sensing Level sensing is standard Requires conductive tips (black, carbon impregnated) Limits tip immersion More accurate pipetting

Software interface Intuitive layout Multiple options to suit user Prepare master mix from reagents or add pre-prepared master mix Dilute Standards each time or use pre-prepared dilutions Normalize sample concentrations Import/Export of sample names (from CSV or tab delineated file or barcode) Wide range of plates can be used (384-, 96-, 72-, Gene-Disc etc)

Compatible with 1.5 ml tapered tubes sample format Compatible with Corbett X-tractor sample trays Compatible with 0.2ml tubes

Rotor-Gene™ Gene-Disc™ Vertical tube orientation means a robot can set up all reactions directly into Gene-Disc tubes The heat sealer provides a permanent or removable film seal (user selectable switch) Gene-Disc rotor ready for cycling

Position and height Before using the robot each block must have definitions for position then height Once you have specified the position to the middle of well 1 on a selected plate all other wells are automatically calibrated Height is defined either using either automatic or manual calibration Recommend checking height automatically first then repeat manually, especially if using small volumes If unsure do not change position and height as this will affect all users

Plate position Select “Options” on the toolbar, then “Robot Setup , Calibrate plate positions Choose plate then use X and y adjustments to select the middle of the first well

Plate height Select “Options”, “Robot Setup”, Calibrate plate heights” Warning comes up to make sure you know what you are doing Select plate, change 4 to 1, click autodetect Repeat, change 4 to 1, and use manual Setup, lower tip until it feels tighter

Sample names and IDs can be imported from CSV or tab delineated files (also excel)

Starting the run Click green arrow button on top toolbar

During the run

After the run

Error Messages

Tip usage options Robot can re-use tips when dispensing mastermix Tip can be re-used 96 times if required We recommend re-use 8-12 times Tip re-use is less precise than single use tips Tips can’t be re-used for samples going into a wet well Robot programmed not to allow a tip that has been in a wet well (e.g. with MM) to be re-used If pipetting sample into a dry well the same tip can be used

Jennifer McMahon 0414 278 369 www.corbettresearch.com Phone: 02 9736 1320 FAX: 02 9736 1364 Freecall: 1800 803 915

20 µL reaction volume, 18 replicates Rotor-Gene™ 3000 results 20 µL reaction volume, 18 replicates Hand pipetting CT std dev 0.07 CAS-1200 robot CT std dev 0.05

15 µL reaction volume, 18 replicates Rotor-Gene™ 3000 results 15 µL reaction volume, 18 replicates Hand pipetting CT std dev 0.11 CAS-1200 robot CT std dev 0.07

10 µL reaction volume, 18 replicates Rotor-Gene™ 3000 results 10 µL reaction volume, 18 replicates Hand pipetting CT std dev 0.12 CAS-1200 robot CT std dev 0.10

5 µL reaction volume, 18 replicates Rotor-Gene™ 3000 results 5 µL reaction volume, 18 replicates Hand pipetting CT std dev 0.64 CAS-1200 robot CT std dev 0.12