PRESS F1 FOR GUIDEANCE Bacteriology 5th Practical MFSH 2003

Contents Haemophilus, Mycobactria , Corynbacteriaua & Bordetella Station 1: Gram stain of Haemophilus 4 Station 2: Gram stain of Haemophilus (pus) 5 Station 3: X,V factors & growth 6 Station 4: Growth of H.Influenzae on agar 7 Station 5: Satellitism 8 Station 6: Treatment & diseases 9 Station 7: Summary 10 Mycobacteria 11 Station 8: Ziehl-Neelsen stain 12 Station 9: Lowenstien Jensen media 13 Station 10: Treatment & diseases 14 Station 11: Summary 15 Cornybacteria 16 Station 12: Gram stain of Cornybacteria 17 Station 13: Albert stain of Cornybacteria 18 Station 14: Media used 19 Station 15: ELEK’s plate 20 Station 16: Treatment & diseases 21 Station 17: Summary 22 Bordetella 23 Station 18: Laboratory diagnosis 24 Station 19: Tratment & diseases 25

Haemophilus

It can look like Bacillus or may look like coccus Station 1 It can look like Bacillus or may look like coccus Coccobacilli

Station 2

Some of them require X & V factors ( Haemophilus influenzae) Station 3 Different species of “Haemophilus” have different requirements to grow and make colonies. Some of them require X & V factors ( Haemophilus influenzae) Some of them need X factor only (Haemophilus ducreyi) Some of them need V factor only (Haemophilus parainfluenzae) X factor = heamatin V factor = Nicotinamide, Adenine & Dineiceotide This Bacteria grow when there are V & X factors. E.g. (H. Influenzae) This Bacteria grow when there is V factor. E.g. (H. Parainfluenzae)

Station 4 Only X factor X+V factors No growth Growth No growth

Station 5 X Factor present This is called Satellitism Staphylococcus strip Producing V Factor H.Influenzae growing here because of presence of both X & V factors

Station 6 Haemophilus influenzae Infection:- respiratory tract infections No bacteremia Haemophilus influenzae Infection:- meningitis acute epiglotitis in children (sore throught) osteomylitis & arthritis Cause bacteremia Treatment:- “Ampicilin” if beta lactamase producers  “Chloramphenicol” Vaccination:- Present by Polysaccharide capsule “serotype b”

Grows on Chocolate agar Station 7 Summary Test yourself H. influenzae H. Parainfluenzae Gram stain Type Needs (X,V factors) Grows on Chocolate agar Grows on Blood agar -ve -ve Coccobacilli Coccobacilli X + V V only Yes Yes No No Best wishes

Mycobacteria

Station 8

Station 9

Station 10 Mycobacterium tuberculosis Infection:- 1-TB “lung infection with bloody cough” 2- Meningitis Treatment:- “Pyrazinamide + Isoniazid + Rifampicin”  2 months then, “Isoniazid + Rifampicin”  4 months Vaccination:- B.C.G “Bacille-Calmatte Guerin “10 years protection”

Summary Best wishes Type “of Mycobacterium”: Stain used: Station 11 Summary Type “of Mycobacterium”: Stain used: Medium used: Acid-Fast Bacilli Ziehl-Neelsen Stain Lowenstein-Jensen medium Best wishes

Corynebacteria

Station 12

Station 13

Black color Station 14 Something like this There is growth but this is a wrong plate

Station 15

Station 16 Corynbacterium diphtheriae Infection:- Diphtheria “Bull neck appearance because of pharyngitis” affect: 1- nervous tissue damage (exotoxin) 2- heart muscle damage (exotoxin) 3- laryngeal obstruction 4- affect skin No bacteremia Treatment:- “Penecilin” Vaccination:- Present by toxoid “DPT vaccine” *D= diptheria toxoid *P= pertussis *T= tetanus

Summary Best wishes Type & “gram” stain: Stains used: Gram +ve bacilli Station 17 Summary Type & “gram” stain: Stains used: Media used: What is used for toxogenity test? Gram +ve bacilli Gram & Albert Potassium tellurite & Loeffler’s ELEK’s plate Best wishes

Bordetella

Station 18 (coocobacili)

Station 19 Bordetella pertussis Infection:- Whooping cough “due to an endotoxin” usually 1 year of age complications: bronchopneumonia, bronchiatasis & subconjuctival hemorrhage Treatment:- “Erythromycin” but only affective at early stages if beta lactamase producers  “Chloramphenicol” No bacteremia Vaccination:- Present by whole killed bacteria “DPT”

اللهم إنا نسألك زيادة في الدين وبركة في العمر وصحة في الجسد وسعة في الرزق وتوبة قبل الموت وشهادة عند الموت ومغفرة بعد الموت وعفوا عند الحساب وأمانا من العذاب ونصيبا من الجنة وارزقنا النظر إلى وجهك الكريم دعواتكم يا شباب

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