A National Science Foundation Engineering Research Center in the MSU College of Engineering Center for Biofilm Engineering INTRODUCTION Evaluation of Fluid.

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Presentation transcript:

a National Science Foundation Engineering Research Center in the MSU College of Engineering Center for Biofilm Engineering INTRODUCTION Evaluation of Fluid Path Colonization in Needle-Free Connectors and Biofilm Formation in Central Venous Catheters E. deLancey Pulcini 1, A.E. Parker 1, M. Ryder 2, G. James 1 (1) Center for Biofilm Engineering Montana State University-Bozeman (2) Ryder Science Inc. Escondido, CA Poster#275 ASM Biofilms 2012 In the United States, approximately 80,000 catheter- related bloodstream infections (CRBSI) occur in ICUs annually 1 and approximately 250,000 CRBSIs occur hospital wide annually 2. Needle-free connectors collected from patients have been shown to contain bacterial biofilms 3. Previous studies have shown bacterial contamination and bacterial transfer through needle-free connectors 4. Needle-free connectors were developed for use with vascular catheters to prevent needle stick injuries to health care workers. The use of needle-free connectors has been implicated in catheter related bloodstream infections. The objective of this study was to determine if there was a difference, by connector type, in the colonization of the fluid pathway in inoculated needle-free connectors and in attached vascular catheters after repeated flushing with no disinfection. Figure 1. The connector-catheter sets were placed in conical vials between flushes. The technician is flushing one of the connector-catheter sets. Figure 3. Image of a triple lumen catheter. For this project, single lumen catheters were used. 1. Mermel LA. Prevention of intravascular catheter-related infections. (Erratum: Ann Intern Med 133:395, 2000). Ann Intern Med :391– Maki DG, Kluger DM, Crnich CJ. The risk of bloodstream infection in adults with different intravascular devices: a systematic review of 200 published prospective studies. Mayo Clin Proc :1159– Donlan RM, Murga R, Bell M, Toscano CM, Carr JH, Novicki TJ, Zuckerman C, Corey LC, Miller JM. Protocol for detection of biofilms on needleless connectors attached to central venous catheters. J Clin Microbiol : Ryder M, Fisher S, Hamilton G, Hamilton M, James G. Bacterial transfer through needlefree connectors: comparison of nine different devices. Presented at the Society for Healthcare Epidemiology of America Annual Scientific Meeting, April 2007 The connector septum was inoculated twice a day with 10 6 CFU Staphylococcus aureus ATCC # The inoculated connector was allowed to dry for 30 minutes and then was attached to a catheter. Each connector-catheter set was flushed with 3.0 ml sterile saline which was collected and plated (First Flush). The catheter-connector sets were sterile saline (SS) flushed twice more, locked with sterile Brain Heart Infusion Broth (BHI) for 1 hour and SS flushed three more times. The last flush was also collected and plated (Last Flush). The connector-catheter sets were inoculated a second time each day after the 6th sterile saline flush followed by a second round of flushing, plating and locks for a total of 18 connector accesses daily, considered to be a routine number of accesses in an intensive care unit. The entire procedure, inoculation and flushing, was repeated each day for 5 days. On Days 4 and 5, two connector-catheter sets for each connector type were destructively sampled for bacterial counts and microscopy. Statistical analysis was performed using mixed effect ANOVA analysis and Tukey’s tests to determine significant mean differences of bacteria in the flush, hub, catheter segment or connector amongst the different needle-free connectors. A multiple linear regression was used to determine if any combination of the variables measured in the study could significantly predict the log density of bacteria either in the flush. METHODSRESULTS REFERENCES RESULTS A total of 8 needle-free connectors were evaluated in this study. Three of each connector type were evaluated in three replicate runs (n=9) with the MicroClave® (ICU Medical Inc.) serving as the matched control for every run (n =27). METHODS ACKNOWLEDGEMENTS This research was funded by ICU Medical. RESULTS Surface Inoculation The mean log densities (LD) of the surface inoculations averaged across all days were statistically equivalent as long as mean differences as large as 0.37 were assumed to be negligible. For each day individually, the mean LD of the surface inoculum (averaged across the two inoculations for each day) were shown to be statistically equivalent as long as mean differences as large as 0.53 were assumed to be negligible. The mean log density (LD) in the daily flush for the MicroClave connector was significantly smaller compared to any of the other connector types tested (p<0.0005). The Q-Syte had the significantly largest mean LD of bacteria in the flush compared to any of the other connector types. (p <0.0005). The MicroClave connector had the significantly smallest mean log density (LD) of bacteria in the flush compared to any of the other connector types (p<0.0005). The mean LD of bacteria in the hub for the MicroClave connector was significantly smaller than the SmartSite, Invision, Q-Syte and Bionector connectors. The mean LD of bacteria in the catheter segment for the MicroClave was significantly smaller that the SmartSite, Invision, Q-Syte and Bionector connectors. The mean LD of bacteria in the MicroClave connector was significantly smaller than the SmartSite, Invision, and Bionector connectors. The daily mean LD of bacteria attached in the hub and in the connector were better predictors of the daily mean LD of bacteria recovered from the flush than the mean LD of bacteria in the catheter segment. The least square mean for all flushes for all days was calculated. The color scheme indicates the significant groups (p< 0.05). The least square mean for the destructive sampling of the catheter hubs was calculated for Days 3 and 4 combined. The color scheme indicates the significant groups (p< 0.05) The least square mean for the destructive sampling of the connectors was calculated for Days 3 and 4 combined. The color scheme indicates the significant groups (p< 0.05). Catheter Hub Catheter Tubing CONCLUSIONS Figure 2. For surface inoculation controls, the connector was swabbed in order to determine the concentration of bacteria on the connector septum. The least square mean for the destructive sampling of the catheter segments was calculated for Days 3 and 4 combined. The color scheme indicates the significant groups (p< 0.05). MicroClave® (ICU Medical Inc.) SmartSite® (CareFusion Corp.) ClearLink ® (Baxter Inc.) Invision® (RyMed Technologies Inc.) Q-Syte  (BD and Co.) Maximus® (MaxPlus) (CareFusion Inc.) One-Link® (Baxter Inc.) Bionector® (Vygon Inc.)