Nitrogen source tracing in the Choptank River Watershed Midshore Riverkeeper Conservancy University of Maryland Center for Environmental Science National.

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Presentation transcript:

Nitrogen source tracing in the Choptank River Watershed Midshore Riverkeeper Conservancy University of Maryland Center for Environmental Science National Fish and Wildlife Foundation

Forms of Nitrogen N2N2

N stable isotopes: how it works

The 15 N: 14 N ratio can vary depending on how nitrogen is used 15 N 14 N Ratio referred to as  15 N

Amount of nitrogen (%N) does not equal  15 N %N  15 N %N  15 N < >

Nitrogen Cycle Atmospheric nitrogen Atmospheric fixation

Nitrogen Cycle Atmospheric nitrogen Atmospheric fixation

Nitrogen Cycle Atmospheric nitrogen Atmospheric fixation Biological fixation

Nitrogen Cycle Atmospheric nitrogen Atmospheric fixation Industrial fixation Biological fixation

Nitrogen Cycle Atmospheric nitrogen Atmospheric fixation Volatilization Industrial fixation Biological fixation

Nitrogen Cycle Atmospheric nitrogen Atmospheric fixation Denitrification Ammonium Nitrite Nitrate Volatilization Industrial fixation Biological fixation

Nitrogen Cycle Atmospheric nitrogen Atmospheric fixation Denitrification Ammonium Nitrite Nitrate Volatilization Industrial fixation Biological fixation

% Nitrogen  15 N Denitrification Animal Waste Volatilization enrichment Fertilizer source Low nitrogen and activity

Objectives Delineate the nitrogen contribution of different activities in the Choptank River Watershed (e.g. poultry, dairy, sewage, cropping, sod, slaughterhouse) Adopt combination of water quality and nitrogen stable isotope composition in aquatic plants and water samples. Relate findings to land-use practices Identify focus areas for further work / targeting of management activities

Sampling Sites Easton STP Cambridge STP Denton STP Cordova Oakland Farm Colliers Farm

What we collected Phragmites along Choptank River –  15 N – %N Grasses in Choptank watershed –  15 N – %N Water quality – MRC – This project

Sample analysis

Denitrification Animal Waste Volatilization enrichment Fertilizer sourceLow nitrogen and activity Results

DenitrificationAnimal Waste Volatilization enrichment Fertilizer source Low nutrients and activity River Watershed Results

DenitrificationAnimal Waste Volatilization enrichment Fertilizer source Low nutrients and activity Opposite Cambridge STP Denton Tuckahoe R. Huntington Ck. Gaineys Wharf Choptank Above Tuckahoe Easton STP Results

DenitrificationAnimal Waste Volatilization enrichment Fertilizer source Low nutrients and activity Opposite Cambridge STP Denton Tuckahoe R. Huntington Ck. Gaineys Wharf Choptank Above Tuckahoe Easton STP Results

DenitrificationAnimal Waste Volatilization enrichment Fertilizer source Low nutrients and activity Oakland (corn – unirrigated /manure) Oakland (corn - irrigated) Oakland Spray irrigated dairy Cordova Ck Trib (poultry, manure, slaughterhouse) Oakland (pond grass) Oakland (manure) Cordova Ck Trib (Downstream Poultry) Colliers (corn – chicken manure) Cordova Ck (downstream slaughterhouse) Masons Branch Trib. Upstream poultry Masons Branch Trib. Downstream poultry Results

DenitrificationAnimal Waste Volatilization enrichment Fertilizer source Low nutrients and activity Oakland (corn – unirrigated /manure) Oakland (corn - irrigated) Oakland Spray irrigated dairy Cordova Ck Trib (poultry, manure, slaughterhouse) Oakland (pond grass) Oakland (manure) Cordova Ck Trib (Downstream Poultry) Colliers (corn – chicken manure) Cordova Ck (downstream slaughterhouse) Masons Branch Trib. Upstream poultry Masons Branch Trib. Downstream poultry Results

Tuckahoe Results

Tuckahoe Results

DenitrificationAnimal Waste Volatilization enrichment Fertilizer source Low nutrients and activity

Results

Oakland Pond Cordova Ck Down. Slaughterhouse Cordova Ck Down. Poultry Cordova Ck Trib (poultry, manure, slaughterhouse) Masons Branch Trib. Upstream poultry Masons Branch Trib. Downstream poultry Results

River Watershed Watershed is distinct whereas river is a mixing bowl

Summary Demonstrated the river is sink of varied catchment activities Management needs to target N and P separately. Make sure any nutrient removal process targets the relevant nutrient. Emphasis on poultry appears to be P and not N Unclear why %N is inverse of TN in the river Shift over the past 10 years in N influence in the Choptank from animal waste to mixed animal waste/fertilizer source.

Next steps… Follow up on the turf farm Investigate above Denton and below Cambridge Consult with other scientists about the inverse relationship with TN and %N (water problem is deposited downstream in the mud???)

Results fix

Results fix