PIGMENT ANALYSIS OF COASTAL WATERS IDENTIFICATION OF MAJOR PIGMENT GROUPS CONTRIBUTING TO THE WATER LEAVING RADIANCE 1.STATISTICAL SEPARATION 2.STRUCTURAL.

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Presentation transcript:

PIGMENT ANALYSIS OF COASTAL WATERS IDENTIFICATION OF MAJOR PIGMENT GROUPS CONTRIBUTING TO THE WATER LEAVING RADIANCE 1.STATISTICAL SEPARATION 2.STRUCTURAL AND SPECTROSCOPIC IDENTIFICATION 3.HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 4.GROUPING BY PLANKTON SPECIES (ChemTax) 5.EXTENSION OF STATISTICS TO LARGE AREA COVERAGE

PECONIC RIVER TEST SITE NUTRIENT INJECTION

HPLC: PIGMENT NOMENCLATURE (SeaBASS) Chl_c3Chlorophyll c3 Chl_c2Chlorophyll c2 Chl_c1c2Chlorophyll c1 + chlorophyll c2 PeridPeridinin But-fuco19’-Butanolyoxyfucoxanthin FucoFucoxanthin Hex-fuco19’-Hexanoyloxyfucoxanthin PrasPrasinoxanthin ViolaViolaxanthin DiadinoDiadinoxanthin AlloAlloxanthin DiatoDiatoxanthin LutLutein ZeaZeaxanthin Chl_bChlorophyll b Chl_aChlorophyll a Beta-epi-CarAlpha carotene Beta-beta-CarBeta carotene Tot_Chl_aMonovinyl chlorophyll a + Divinyl chlorophyll a + Chlorophilide a + Chlorophyll a allomer + Chlorophyll a epimer

SPECTRAL RESEMBLANCE OF CAROTENOIDS More than one pigment can have the same absorption spectra, so retention time becomes important in discriminating between pigments. Alloxanthin…… λ max = 426, 454, 483 nm Diatoxanthin….. λ max = 427, 454, 482 nm Zeaxanthin……. λ max = 428, 455, 483 nm β-carotene…….. λ max = 433, 455, 482 nm

PIGMENT IDENTIFICATION

SATELLITE PIGMENT ESTIMATES CORRELATION/ CALIBRATION YEARLY HISTORICAL MEAN IN SITU SATELLITE YEARLY MEAN 670/680 nm QUALITY CHECK APPLY ALGORITHM TO SINGLE ORBITS COMPARISON WITH SHIPS RECALIBRATE CONTOUR MAP σ CONTOUR MAPS WITH STANDARD DEVIATIONS

MEAN ANNUAL SEA SURFACE TEMPERATURE

MEAN ANNUAL CHLOROPHYLL (mg · m -3)

BAND RATIO 678nm/667nm Day 284, 2001

STRUCTURAL SIMILARITY OF PHOTOSYNTHETIC CAROTENOIDS  -CAROTENE PERIDININ FUCOXANTHIN

UPWARD RADIANCE OF DIFFERENT WATER TYPES