Purple Non Sulfur Bacteria

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Presentation transcript:

Purple Non Sulfur Bacteria Hopkins Microbiology Course Ann Lesnefsky 7-15-2011

Metabolism of PNS Bacteria Organic acid electron donor Photosynthesis for energy generation Bacterial chlorophyll b Absorbs light in the infrared range Currently PNS bacteria are being studied for H2 production

Course Enrichment Method for PNS 6-18-2011 Plated samples from the slough on succinate and ethanol plates, incubated anaerobically with light Course’s media and Holly’s media 6-29-2011 Restreak single colonies for isolation

TA Enrichment Method for PNS TA’s enriched for PNS by filtering Filter both water above mat and slurry of mat material Place filter face down on plate Flip and all allow PNS bacteria to grow on filter

Many Some Few Carbon Source Enrichment Method Succinate Ethanol (# of colonies before restreak) Ethanol No filter Course 43 6 Holly Filter Many Some Few None

Microscopy of PNS Colonies isolated from 5 different plates 4 plates from strawberry 1 from Kerby Park All looked like stubby rods

Recommendations Next year use the filter method.

Questions?