A Review of the 2014 Mark Procedures Manual Tiffani Marsh 1, Charles Morrill 2, Pat Keniry 3, Stephen Pastor 4, Jeff Fryer 5, Scott Putnam 6, Brandon Chockley 7 1. NOAA Fisheries; 2. Washington Department of Fish & Wildlife 3. Oregon Department of Fish & Wildlife 4. U.S. Fish and Wildlife Service 5. Columbia River Intertribal Fish Commission 6. Idaho Department of Fish & Game 7. Fish Passage Center

Outline 1.Purpose of the manual

Outline 1.Purpose of the manual 2.Special Notices

Outline 1.Purpose of the manual 2.Special Notices 3.Review of the various sections

Outline 1.Purpose of the manual 2.Special Notices 3.Review of the various sections 4.Questions

Purpose As stated in the document This PIT Tag Marking Procedures Manual provides protocols and standards on the PIT tag marking station(s), fish handling techniques, anesthesia, tag injection, and information on data collection, verification, and transfer to the regional database. In addition to this manual, the PTSC and PTAGIS produced a PIT tag training video that is available from PTAGIS. If you have specific questions that are not answered by this document or the video, or if you would like to receive training, please contact a PTSC representative for assistance.

Purpose As stated in the document This PIT Tag Marking Procedures Manual provides protocols and standards on the PIT tag marking station(s), fish handling techniques, anesthesia, tag injection, and information on data collection, verification, and transfer to the regional database. In addition to this manual, the PTSC and PTAGIS produced a PIT tag training video that is available from PTAGIS. If you have specific questions that are not answered by this document or the video, or if you would like to receive training, please contact a PTSC representative for assistance. PIT Tag Specification Document

Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology.

Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on

Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags

Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag

Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag water temperature

Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag water temperature fish size

Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag water temperature fish size Juvenile salmonid tagging

Purpose This manual provides guidelines developed from 20 + years of experience with PIT tag technology. The guidelines are based on Use of 12mm FDX tags Having the ability to choose when to tag water temperature fish size Juvenile salmonid tagging It is understood that some research studies will not be able to follow some of the protocols.

Special Notices Since July 1992, only disinfected injectors and tags have been approved for use in the Columbia River Basin for all hatchery and wild anadromous fish. This requirement eliminated the use of semiautomatic injectors. Disinfection using standard hand held injectors requires immersing the needle, syringe, and the end of the injector push rod in 70-80% ethyl alcohol or 60-80% isopropyl alcohol for a period of not less than 10 minutes. Currently, there is a single-use injector system (SUI) available to which the disinfection requirement does not apply, as long as the needle assembly used in this system is not re-used. If a tagger wishes to re-use the single-use needle assembly, disinfection between fish is required.

Special Notices Since July 1992, only disinfected injectors and tags have been approved for use in the Columbia River Basin for all hatchery and wild anadromous fish. This requirement eliminated the use of semiautomatic injectors. Disinfection using standard hand held injectors requires immersing the needle, syringe, and the end of the injector push rod in 70-80% ethyl alcohol or 60-80% isopropyl alcohol for a period of not less than 10 minutes. Currently, there is a single-use injector system (SUI) available to which the disinfection requirement does not apply, as long as the needle assembly used in this system is not re-used. If a tagger wishes to re-use the single-use needle assembly, disinfection between fish is required.

Special Notices The PTSC and FPAC members do not endorse or recommend any tagging location in anadromous salmonids other than what is described in this manual. Tagging in any location other than the abdominal cavity increases the risk of human consumption.

Special Notices The PTSC and FPAC members do not endorse or recommend any tagging location in anadromous salmonids other than what is described in this manual. Tagging in any location other than the abdominal cavity increases the risk of human consumption. Fisherman in the lower river – dorsal sinus

Special Notices The PTSC and FPAC members do not endorse or recommend any tagging location in anadromous salmonids other than what is described in this manual. Tagging in any location other than the abdominal cavity increases the risk of human consumption. Fisherman in the lower river – dorsal sinus Idaho Statesman article, A pit tag is crunchy, but not tasty, 11 March 2014

Contents

1.Tagging stations

Contents 1.Tagging stations 2.Tagging operations

Mass marking

Traps and weirs

Streamside

Contents 3.Fish handling techniques

Contents 3.Fish handling techniques Minimizing Fish Stress

Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding

Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers

Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers Anesthesia

Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers Anesthesia - concentration, stock solution, anesthetization

Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers Anesthesia - concentration, stock solution, anesthetization 4.Fish Recovery and Release

Contents 3.Fish handling techniques Minimizing Fish Stress - fish health, water temperature, oxygen, crowding Marking and recovery containers Anesthesia - concentration, stock solution, anesthetization 4.Fish Recovery and Release 5.Post-Tagging Mortality and Tag Retention

Contents 6.PIT Tag Injectors

Contents 6.PIT Tag Injectors Multiple use injectors (MUI)

Contents 6.PIT Tag Injectors Multiple use injectors (MUI) Disinfection

Contents 6.PIT Tag Injectors Multiple use injectors (MUI) Disinfection Construction

Contents 6.PIT Tag Injectors Multiple use injectors (MUI) Disinfection Construction Single use injectors (SUI)

Contents 6.PIT Tag Injectors Multiple use injectors (MUI) Disinfection Construction Single use injectors (SUI) Exempt from disinfection, unless re-used

Contents 7.Tag Injection

Holding the fish

Holding the injector

Tag insertion

Tag placement

Contents 7.Tag Injection Holding the fish Holding the needle Tag insertion Tag placement Fish size

Contents 7.Tag Injection Holding the fish Holding the needle Tag insertion Tag placement Fish size 65mm or larger

Contents 7.Tag Injection Holding the fish Holding the needle Tag insertion Tag placement Fish size 65mm or larger 8.Computerized Data Entry

Possible additions to document Guidelines Smaller tags Tagging adult salmonids Tagging non-salmonids Additional anesthetics

Web links – PTAGIS library - this manual and 2009 Specification Doc library – BPA Details/ Chris Tatara Details/ Details/ Tiffani Marsh Details/4146

Questions?