Apaf-1 Caspase-9 Cyt c control sulindac control E LY control TRAIL thapsigargin HT-29Chondrocyte control SIN-1 RAW IP: AChE C Supplementary Fig. S1 AB IP : Apaf-1 WB: AChE IP: Caspase-3 WB: AChE IP: Caspase-9 WB: AChE IP: c-IAP-1 WB: AChE IP: c-IAP-2 WB: AChE IP: Cyt c WB: AChE E treated Thapsigargin 2.5 M, 24 h HT-29 Sulindac 1 mM, 48 h E40 40 h LY M, 24 h TE671 BMMC U373MG E50 24 h E40 24 h SK-MEL5 E10 48 h RAW SIN-1 1 mM, 24 h IL-3 deprivation Malme-3M E50 24 h TRAIL 100 MOI, 72 h Chondrocyte SNP 1.5 mM, 24 h -actin control treated
E40 si Cyt c Cyt c Hoechst A Supplementary Fig. S2 B si Cyt c h Chondrocyte TRAIL 100 MOI HT-29 Thapsigargin 2.5 M Apaf-1 IP: AChE IgG IP: Apaf-1 WB: Apaf-1 IP: Apaf-1 WB: Apaf-1 IgG PC IP: AChE SK-MEL-5 E10 RAW SIN-1 1 mM h Apaf-1 IgG si Cyt c IP: Apaf-1 WB: Apaf-1 IP: Apaf-1 WB: Apaf-1 PC
Supplementary Fig. S3 IP : Cav-1 E h AChE IgG A B si Cyt c E h IP: Cav-1 IgG AChE C D IP: Apaf-1 WB: AChE E50 (LNCaP)E10 (PC-3) h IP: Cav-1 WB: AChE IP: Cyt c WB: AChE IP: Cyt c WB: Apaf-1 IP: Apaf-1 WB: AChE IP: Cav-1 WB: AChE IP: Cyt c WB: AChE IP: Cyt c WB: Apaf-1 IP: AChE WB: AChE IP: AChE WB: AChE IP: AChE WB: AChE IP: Apaf-1 WB: Apaf-1 IP: AChE WB: AChE IP: AChE WB: AChE IP: AChE WB: AChE IP: Apaf-1 WB: Apaf-1 PC IgG E h IgG Apaf-1 Cyt c IP: Cav-1 IP: Apaf-1 WB: Apaf-1 IP: Cyt c WB: Cyt c IgG PC E IP: Cav-1 M CD E / h AChE IP: Apaf-1 AChE Cyt c IgG IP: Cyt c