Radiation-resistance? There's a Gene for That. New York City Wetware iGEM Team.

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Presentation transcript:

Radiation-resistance? There's a Gene for That. New York City Wetware iGEM Team

Extraordinary Biology Numbers Extremophile Numbers Extraordinary Synthetic Biology Numbers 130 Celsius 130 MPa 1.08-mega-base pair4 Days

Deinococcus radiodurans (D. rad) One to two hours of acute six to ten Gray radiation exposure. 12,000 Gray!!!!!

Life of the Cell

A Bad Day Base DamageSingle Strand BreakDouble Strand Break Active Site Damage

The Extraordinary Weapon (Kryptonite) 1.H 2 O  H· + OH· 2.OH· + OH·  H 2 O 2 3.Fe 2+ + H 2 O 2 → Fe 3+ + OH· + OH − Generation of Hydroxyl Radical via the Fenton Reaction

What Makes D. rad So Special? 1.Cellular Manganese-Iron Ratios 1.Neutralization of Superoxide Radicals 1.Superior DNA Repair Three Basic Approaches

What Makes D. rad So Special? 1.Cellular Manganese-Iron Ratios Three Basic Approaches

What Makes D. rad So Special? 2.Neutralization of Superoxide Radicals Three Basic Approaches

What Makes D. rad So Special? 3.Superior DNA Repair Three Basic Approaches recA Repair Protein

Making E. coli Radioresistant

Our Biobricks ruvA ruvB radA tpx ruvA

Future Applications Bioremediation

Future Applications Medical Applications

in vitro meat and moral traditions

Kosher Vegetarian Vegan

Legal Precedent: Gelatin

Legal Precedent: Rennet

Moral Considerations Ought a vegan or vegetarian to eat meat? Ought a kosher-observer to eat pork? Does cultivating meat without encountering an animal desensitize us as eaters? Does it undermine the relationship between ourselves and animals?

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