MLAB 1415: Hematology Micros 60

Micros 60 3-part differential Sample size= 10µL Environment Indoor only Level work station Avoid direct exposure to sunlight Proper ventilation 60-90oF Linearity (AMR) WBC (103/mm3): 0-100 RBC (106/mm3): 0-8.0 Hgb (g/dL): 2.0-27 Hct (%): 1.8-82.3 Plt (103/mm3): 25-4990

Parameters Parameters Measurements/Calculations WBC/RBC/PLT Hgb Hct MCV MCH MCHC RDW Platelet MPV Lym # Lym% Mono # Mono% Gra# Gra% WBC/RBC/PLT Impedance change HGB Spectrophotometry LYM%/MON%/GRA% Calculations MCV, MCH,MCHC,RDW,MPV,LYM#, MON#, GRA#

Stats Principle of operation: The counting principle used for the red blood cells, white blood cells and platelets is based on the variations in impedance generated by the passage of cells through a calibrated aperture. Number of samples which can be analyzed per hour: 60 Number of tests performed on this analyzer: 1 Quality control: Three levels run each day of patient testing, or following instrument repair, reagent lot change, or when indicated. Calibration: Shift in control values, replacement of instrument parts, reagent number change, or when indicated Preventative maintenance: Daily: start up and shut down, general cleaning Acceptable samples: whole blood from an EDTA tube ran within 4 hours of collection Limitations: Lipemia, cold agglutinins, high WBC’s

Equipement 8 Main parts Electrical supply Electronic board Dilution pneumatics Control panel with keyboard and LCD screen Reagent compartment Printer Smart card reader Bar code reader (optional)

Reagents Waste Diluent Cleaner LYSE: potassium cyanide ABX minidil: sodium hydroxide/azide Determines and differenciates blood cells Cleaner ABX miniclean: enzymatic detergent Cleans system LYSE: potassium cyanide ABX Alphalyse: Lyses RBCs, releasing hemoglobin and WBCs. WBCs are counted by impedance. Waste

Distribution curves WBC RBC Plt

Daily Start Up Turn on instrument, once on, the start-up will begin automatically Instrument is performing a cleaning and rinsing cycle: detergent in the chamber is rinsed with diluent Cycle lasts about 130 seconds At cycle completion, background values will be displayed. These values must be less than the below: WBC < 0.3 x 103/mm3 RBC < 0.02 x 106/mm3 Hgb < 0.0 g/dL Plt < 10x 103/ mm3 If the values are not within this range, another cycle will begin. If still not in range, the Micros will do another start-up. After 3 failed cycles, it will give a “start-up failed” message. Troubleshooting will now start.

Daily start-up con’t Run a primer sample Perform QC and evaluate Complete maintenance sheet If all QC in, can continue with patient samples.

Running a Patient Press the ID button on the key pad Manually enter the ID. Use the up and down arrows to enter alphabetical characters. Please Enter after each entry.

Shutdown Hit the Stand by key, wait 1 minute Switch off instrument and printer. When the shutdown hit is pressed, the Micros 60 performs a complete cleaning with the Miniclean and puts the system into a Standby mode. Once this process is complete, the instrument can be switched off.